https://eln.excellgen.com/index.php?action=history&feed=atom&title=2374_pFastbac-oriP 2026-10-18T10:47:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2374_pFastbac-oriP&diff=13664&oldid=prev 2014-11-26T19:27:59Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2374 pFastbac-oriP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2374 pFastbac-oriP-&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-9---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2374&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 371 pCEP4 E02S CK2M EN2L&lt;br&gt;2374-oriP-ENBA-Xho1-R&lt;br&gt;(CTTGTCCTTTTCCTGCTGGTTG CTCgag tactaa)&lt;br&gt;ttagtactcGAGCAACCAGCAGGAAAAGGACAAG&lt;br&gt; <br /> <br /> 2374-oriP-ENBA-Kpn1-F&lt;br&gt;tagcacGGTACCTGTAATCCCTTCAGTTGGTTG <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2321 2374PCR.JPG]]&lt;br&gt; <br /> <br /> Digest with Kpn1-Xho1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2321 2374.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_9&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp;Kpn1-Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2321 2374digest.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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