https://eln.excellgen.com/index.php?action=history&feed=atom&title=2378_pT7A3-Cre 2026-10-18T23:55:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2378_pT7A3-Cre&diff=13754&oldid=prev 2014-12-26T18:01:06Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of2378 pT7A3-Cre&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2378 pT7A3-Cre-&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-11---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2378&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR Cre from 1708 pT7A3-HTNCre <br /> <br /> 2378-Cre-Nco1-F tagcaaccatgggcagcagctccaatttactgaccgtacac &lt;br&gt; <br /> <br /> 2378-Cre-TEV-Nde1-R (gcctgctggaagatggcgatGGTACCggggatgacgatgacaaggaaaacctttacttccagggcCAtatgaacac) gtgttcataTGgccctggaagtaaaggttttccttgtcatcgtcatccccGGTACCatcgccatcttccagcaggc <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2378 2382PCR.JPG]]&lt;br&gt; <br /> <br /> Digest with Nde1-Nco1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2378 2382 2384PCR1.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_11&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp;Nde1-Nco1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2378 2382 2384DIGEST.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

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