https://eln.excellgen.com/index.php?action=history&feed=atom&title=2381_2383_2386_expression_screening_2375_purification 2381 2383 2386 expression screening 2375 purification - Revision history 2026-10-18T15:05:51Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2381_2383_2386_expression_screening_2375_purification&diff=19577&oldid=prev Paul.xu at 16:43, 8 November 2018 2018-11-08T16:43:40Z <p></p> <p><b>New page</b></p><div>== Protein Expression ==<br /> <br /> 2014/11/24&lt;br/&gt;SHANYI<br /> <br /> [[File:2381 2383 2386 2375p.jpg|RTENOTITLE]]<br /> <br /> Nico21(DE3)plysN 0.2mM IPTG RT induce overnight<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | MW, PI<br /> | &lt;br/&gt;<br /> | Expression<br /> |-<br /> | 1<br /> | 2381&lt;br/&gt;<br /> | pT7A3-TRX-Tat-NLS-iFlp-6H&lt;br/&gt;<br /> | 9.32 / 66.1&lt;br/&gt;<br /> | supernatant&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 2<br /> | 2381&lt;br/&gt;<br /> | pT7A3-TRX-Tat-NLS-iFlp-6H&lt;br/&gt;<br /> | 9.32 / 66.1&lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | Y<br /> |-<br /> | 3<br /> | 2383&lt;br/&gt;<br /> | pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H&lt;br/&gt;<br /> | 9.49 / 65.0&lt;br/&gt;<br /> | supernatant&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 4<br /> | 2383&lt;br/&gt;<br /> | pT7A3-6XHis-SMT3-Tat-NLS-iFlp-6H&lt;br/&gt;<br /> | 9.49 / 65.0&lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | Y<br /> |-<br /> | 5<br /> | 2386&lt;br/&gt;<br /> | pET30B-T4-uvsX&amp;nbsp;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | supernatant&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 6<br /> | 2386&lt;br/&gt;<br /> | pET30B-T4-uvsX&amp;nbsp;&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 7<br /> | &lt;br/&gt;<br /> | PageRuler Plus&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 8<br /> | 2375&lt;br/&gt;<br /> | pT7A3-MBP-TRX-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | supernatant&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 9<br /> | 2375&lt;br/&gt;<br /> | pT7A3-MBP-TRX-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | supernatant&lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 10<br /> | 2375&lt;br/&gt;<br /> | pT7A3-MBP-TRX-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | Y<br /> |-<br /> | 11<br /> | 2375&lt;br/&gt;<br /> | pT7A3-MBP-TRX-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | Y<br /> |-<br /> | 12<br /> | 2375&lt;br/&gt;<br /> | pT7A3-MBP-TRX-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | pellet&lt;br/&gt;<br /> | Y<br /> |}<br /> <br /> <br /> <br /> <br /> [[Category:Protein Purification]]</div> Paul.xu