https://eln.excellgen.com/index.php?action=history&feed=atom&title=2382_pT7A3-6XHis-SMT3-%28Tev%29 2026-10-18T22:30:05Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2382_pT7A3-6XHis-SMT3-(Tev)&diff=13675&oldid=prev 2014-11-27T17:46:58Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of2382 pT7A3-6XHis-SMT3-(Tev)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2382 pT7A3-6XHis-SMT3-(Tev)&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-11---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2382&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> CR from 2285 pBY011-SMT3 <br /> <br /> 2197-SUMO-Nco1-F1 tcgac cc atg ggc agc agc cac cat cac cat cac cat tcggactcagaagtcaatc <br /> <br /> 2382-SMT3_TEV_Nde1-R (cacagagaacagattggtggt ggggatgacgatgacaaggaaaacctttacttccagggccatatg cat atg tagcac) gtgctacatatgcatatggccctggaagtaaaggttttccttgtcatcgtcatccccaccaccaatctgttctctgtg <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:2378 2382PCR.JPG]]&lt;br&gt; <br /> <br /> Digest with Nde1-Nco1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2378 2382 2384PCR1.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_11&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp;Nde1-Nco1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2378_2382_2384DIGEST.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div>

Excellgen