https://eln.excellgen.com/index.php?action=history&feed=atom&title=2384_pT7A3E-Apyrase 2384 pT7A3E-Apyrase - Revision history 2026-10-18T23:20:30Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2384_pT7A3E-Apyrase&diff=13822&oldid=prev Excellgen at 18:19, 8 January 2015 2015-01-08T18:19:08Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of2384 pT7A3E-Apyrase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2384 pT7A3E-Apyrase&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-11---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2384&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> gene synthesis <br /> <br /> [[Image:DIGEST FOR 2378 2382 2384PCR1.JPG]]&lt;br&gt; <br /> <br /> join two fragments by pcr <br /> <br /> [[Image:2384PCR.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> Digest with Nde1-Xho1&lt;br&gt; <br /> <br /> [[Image:DIGEST FOR 2384.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_11&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp;Nde1-Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2378 2382 2384DIGEST.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Cloning of2384 pT7A3E-Apyrase&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2384 T7A3E-Apyrase&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2014-12---- <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2384&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> subclone from 2380 Digest with Nde1-Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for 2388 2389 2390-1.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2014_11&lt;br&gt; <br /> <br /> Make miniprep, use&amp;nbsp;Nde1-Xho1 to find positive clone&lt;br&gt; <br /> <br /> [[Image:2384 2388 2389 2390digest.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen