https://eln.excellgen.com/index.php?action=history&feed=atom&title=2401_pET30b-scRAD51 2401 pET30b-scRAD51 - Revision history 2026-10-18T12:07:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2401_pET30b-scRAD51&diff=13935&oldid=prev Excellgen at 16:21, 6 February 2015 2015-02-06T16:21:35Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2401 pET30b-scRAD51&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2401 pET30b-scRAD51&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015_1 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2401&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR from 2396&lt;br&gt; <br /> <br /> 1.&lt;br&gt;2401-scRAD51-Nde1-F&lt;br&gt;tactaacatatggcgtttttaagctattttg&lt;br&gt;2401-RAD51-R&lt;br&gt;cttctagcatgtgaggccccag <br /> <br /> &lt;br&gt; <br /> <br /> 2.&lt;br&gt;2401-scRAD51-F&lt;br&gt;ctggggcctcacatgctagaag <br /> <br /> 2401-scRAD51-Xho1-R&lt;br&gt;(ctgcatgcacgcaagcctact ctcgag tatcac)&lt;br&gt;gtgatactcgagagtaggcttgcgtgcatgcag <br /> <br /> [[Image:2400 2394 2401-2 2403pcr.JPG]][[Image:2321 2399 2400 2401-1.JPG]] <br /> <br /> 3. <br /> <br /> 2401-scRAD51-Nde1-F&lt;br&gt;tactaacatatggcgtttttaagctattttg <br /> <br /> 2401-scRAD51-Xho1-R&lt;br&gt;(ctgcatgcacgcaagcctact ctcgag tatcac)&lt;br&gt;gtgatactcgagagtaggcttgcgtgcatgcag&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> [[Image:758fix 760fix 2401 2321 2399pcr.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> digest with Nde1 + Xho1&lt;br&gt; <br /> <br /> [[Image:Digest for 758fix 760fix 2394 2404 2400 2401 2402 2403.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use Nde1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:758fix 760fix 2394 2400 2401 2402 2404 2403.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === CLONING ===<br /> <br /> PCR from 2396&lt;br&gt; <br /> <br /> 2401-scRAD51-Nde1-F tagataccATG ggt TCTCAAGTTCAAGAACAACATATATC<br /> <br /> 2401-scRAD51-Xho1-R (GACCCCAGAGAAGAAGACGAG ctcgag tagcac) gtgctactcgagCTCGTCTTCTTCTCTGGGGTC <br /> <br /> [[Image:Digest_for2401_1881_2413pcr.JPG]]&lt;br&gt; <br /> <br /> digest with Nco1 + Xho1&lt;br&gt; <br /> <br /> [[Image:1881_766_2401.JPG]]<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use Nco1 + Xho1 to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:2401digest.JPG]]&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen