https://eln.excellgen.com/index.php?action=history&feed=atom&title=2402_pEC-miraculin 2402 pEC-miraculin - Revision history 2026-10-18T14:09:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2402_pEC-miraculin&diff=13840&oldid=prev Excellgen: Created page with "== '''Cloning of 2402 pEC-miraculin''' == '''Purpose:''' Cloning of 2402 pEC-miraculin'''==''' '''Date: '''2015_1 '''Notebook:0''' '''Page:''' '''Clone ID:2402''' '''..." 2015-01-13T21:26:17Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of 2402 pEC-miraculin&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2402 pEC-miraculin&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2015_1 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ID:2402&#039;&#039;&#039; &#039;&#039;&#039;...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2402 pEC-miraculin&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2402 pEC-miraculin&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015_1 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2402&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> <br /> <br /> gene synthesis<br /> <br /> <br /> <br /> &lt;br&gt; [[Image:2394_2404_2402pcr.JPG]]<br /> <br /> digest with Nde1 + Nco1&lt;br&gt; <br /> <br /> [[Image:Digest for 758fix 760fix 2394 2404 2400 2401 2402 2403.JPG]] <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> Make miniprep, use Nde1 + Nco1 to find positive clone&lt;br&gt; <br /> <br /> &lt;br&gt; [[Image:758fix 760fix 2394 2400 2401 2402 2404 2403.JPG]]&lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen