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2406 pEC-35S-Miraculin - Revision history
2026-10-18T20:59:21Z
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Excellgen at 17:13, 18 March 2015
2015-03-18T17:13:35Z
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<p><b>New page</b></p><div>== '''Cloning of '''<span dir="auto">406 pEC-35S-Miraculin</span ==<br />
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'''Purpose:''' Cloning of <span dir="auto">406 pEC-35S-Miraculin</span><br />
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'''Date:&nbsp;''' 2015-3-13<br />
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'''Notebook:&nbsp;'''<br />
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'''Page:&nbsp;'''<br />
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'''Clone ID: 2412'''<br />
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'''Experimenters:''' Paul & Xu Xin<br />
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== '''Results''' ==<br />
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=== cloning ===<br />
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Date: 3/14/2015<br />
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Digest plasmid DNA with Nde1 + EcoR1<br />
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combine insert from 2403 and digested vector 2402, purify, ligate, transform DH5alpha<br />
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[[File:2406 3-16-2015.JPG|RTENOTITLE]]<br />
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screeing 6 colony from transformation plate, mini-pre, digestion, screening. All the 6 colonies have the product. make the glycerol stock as 2406 use the first digstion plasmid. For the plasmid stock&nbsp; 2406 with the plasmid#1; and mix all the other plasmids make '''2406 mix'''<br />
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== Colony Screening ==<br />
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Date: <br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Excellgen