https://eln.excellgen.com/index.php?action=history&feed=atom&title=2410_pFastBac-VSVG-CMV5-Flp 2410 pFastBac-VSVG-CMV5-Flp - Revision history 2026-10-18T14:09:52Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2410_pFastBac-VSVG-CMV5-Flp&diff=13888&oldid=prev Excellgen: Created page with "== '''Cloning of2410 pFastBac-VSVG-CMV5-Flp''' == '''Purpose:''' Cloning of2410 pFastBac-VSVG-CMV5-Flp'''==''' '''Date: '''2015_1 '''Notebook:0''' '''Page:''' '''Clone ..." 2015-01-26T18:12:08Z <p>Created page with &quot;== &#039;&#039;&#039;Cloning of2410 pFastBac-VSVG-CMV5-Flp&#039;&#039;&#039; == &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2410 pFastBac-VSVG-CMV5-Flp&#039;&#039;&#039;==&#039;&#039;&#039; &#039;&#039;&#039;Date: &#039;&#039;&#039;2015_1 &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; &#039;&#039;&#039;Page:&#039;&#039;&#039; &#039;&#039;&#039;Clone ...&quot;</p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of2410 pFastBac-VSVG-CMV5-Flp&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of2410 pFastBac-VSVG-CMV5-Flp&#039;&#039;&#039;==&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015_1 <br /> <br /> &#039;&#039;&#039;Notebook:0&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:2410&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; shanyi <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === CLONING ===<br /> <br /> PCR CMV promoter from a clone such as 2216 pCMV5-BirA &lt;br&gt;<br /> <br /> 2410-CMV-Sal1-F tagaca GTCGAC GCCCATATATGGAGTTCCGCGTTAC&lt;br&gt;<br /> <br /> 2410-CMV-PH-R GTTATCATTTTAATTATCTCCATAAACTTGGACCTGGGAGTGGACAC <br /> <br /> PCR PH promoter from pFastbac vector &lt;br&gt;<br /> <br /> 2410-CMV-PH-F GTGTCCACTCCCAGGTCCAAGTTTATGGAGATAATTAAAATGATAAC <br /> <br /> 2410-PH-BamH1-R (CATACCGTCCCACCATCGGGCGCGGATCCCGGTC) GACCGGGATCCGCGCCCGATGGTGGGACGGTATG<br /> <br /> [[Image:2387 2409 2410pcr.JPG]]<br /> <br /> Join CMV5 and PH promoter by &lt;br&gt;<br /> <br /> 2410-CMV-Sal1-F tagaca GTCGAC GCCCATATATGGAGTTCCGCGTTAC&lt;br&gt;<br /> <br /> 2410-PH-BamH1-R (CATACCGTCCCACCATCGGGCGCGGATCCCGGTC) GACCGGGATCCGCGCCCGATGGTGGGACGGTATG <br /> <br /> [[Image:2409_2410pcr.JPG]]<br /> <br /> digest vector with Xho1 + BamH1 digest PCR product with Sal1 + BamH1&lt;br&gt;<br /> <br /> [[Image:DIGEST FOR 760 2387 2409 2410.JPG]]&lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2015_1&lt;br&gt; <br /> <br /> &lt;br&gt; Make miniprep, use NCO1 to find positive clone,will get three bands.&lt;br&gt; <br /> <br /> [[Image:2410digest.JPG]]&lt;br&gt; &lt;br&gt; &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> [[Category:Cloning]]</div> Excellgen