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2418-pCold-MBP-(Ent-TEV)-UvsY - Revision history
2026-10-18T17:26:22Z
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Excellgen at 14:23, 6 May 2015
2015-05-06T14:23:43Z
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<p><b>New page</b></p><div>== '''Cloning of<span dir="auto">2418-pCold-MBP-(Ent-TEV)-UvsY</span>''' ==<br />
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'''Purpose:''' Cloning of <span dir="auto">2418-pCold-MBP-(Ent-TEV)-UvsY</span><br />
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'''Date:&nbsp;''' 2015-April<br />
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'''Experimenters:''' Xin<br />
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== '''Results''' ==<br />
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=== '''Cloning''': ===<br />
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Subclone 2241 into vector 2185. As 2185 was used as vector to clone before, no clony.<br />
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So, 2367 (vector as 2185), as used to clone as vector.<br />
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[[File:2418 2434 clone.JPG|381x181px|RTENOTITLE]]<br />
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Gel purification, ligation, transformation and screening.<br />
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Screening Gel by NdeI/ XhoI<br />
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[[File:2418 2434 screening.JPG|303x146px|RTENOTITLE]]<br />
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Clone#4 was the positive one.<br />
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== '''QC:''' ==<br />
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Compare the cutting size between the positive colony#4 and the insert 2241 in one gel.<br />
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[[File:2418 QC.JPG|RTENOTITLE]]<br />
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QC: To verify MBP gene was in the plasmid. Compare with the vector 2185.<br />
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[[File:2418 QC1.JPG|109x203px|RTENOTITLE]]<br />
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Same size of MBP gene was digested on both plasmids.&nbsp;<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
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