https://eln.excellgen.com/index.php?action=history&feed=atom&title=2422_pEC-35S-Miraculin-Term 2422 pEC-35S-Miraculin-Term - Revision history 2026-10-18T23:03:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2422_pEC-35S-Miraculin-Term&diff=14037&oldid=prev Paul.xu at 14:41, 24 March 2015 2015-03-24T14:41:12Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of &#039;&#039;&#039;2422 pEC-35S-Miraculin-Term ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 2422 pEC-35S-Miraculin-Term<br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; 2015-3-21<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 2422&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Paul<br /> <br /> == &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Results&#039;&#039;&#039;&lt;/span&gt; ==<br /> <br /> === &lt;span dir=&quot;auto&quot;&gt;subcloning from&amp;nbsp; 2406&amp;nbsp; pEC-35S-Miraculin to&amp;nbsp; 2420 pEC-Miraculin-Term&amp;nbsp; using EcoR1 + Kpn1&lt;/span&gt; ===<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;Date: 3/21/15&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;1: 2420 pEC-Miraculin-Term, Kpn1 + Nco1&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;2: 2406&amp;nbsp; pEC-35S-Miraculin, EcoR1 + Kpn1&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;3: 2420 pEC-Miraculin-Term, EcoR1 + Kpn1&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;[[File:2422 EcoR1 Kpn1 subcloning 3 21 15.JPG|RTENOTITLE]]&lt;/span&gt;<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;cut out insert from 3, and vector 2, add QG, purify, ligate and transform DH5alpha.&lt;/span&gt;<br /> <br /> <br /> <br /> <br /> <br /> === &lt;span dir=&quot;auto&quot;&gt;Colony Screening&lt;/span&gt; ===<br /> <br /> &lt;span dir=&quot;auto&quot;&gt;Date:&amp;nbsp; 2015&lt;/span&gt;<br /> <br /> == &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;/span&gt; ==<br /> <br /> == &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;/span&gt; ==<br /> <br /> == &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;&lt;/span&gt; ==<br /> <br /> == &lt;span dir=&quot;auto&quot;&gt;&#039;&#039;&#039;Conclusion&#039;&#039;&#039;&lt;/span&gt; ==<br /> [[Category:Cloning]]</div> Paul.xu