https://eln.excellgen.com/index.php?action=history&feed=atom&title=2434-pCold-MBP-%28Ent-TEV%29-T5-Exo2434-pCold-MBP-(Ent-TEV)-T5-Exo - Revision history2026-10-18T14:11:45ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=2434-pCold-MBP-(Ent-TEV)-T5-Exo&diff=14356&oldid=prevExcellgen at 21:39, 4 May 20152015-05-04T21:39:47Z<p></p>
<p><b>New page</b></p><div>== '''Cloning of <span dir="auto">2434-pCold-MBP-(Ent-TEV)-T5-Exo</span>''' ==<br />
<br />
'''''Date:&nbsp;''''''2015-''April''<br />
<br />
== ==<br />
<br />
'''Clone ID'':2434'''''<br />
<br />
'''Experiment''''': '''Xin<br />
<br />
== '''Results''' ==<br />
<div>Subclone 1021 pET8XHis-T5 Exo into vector 2185&nbsp;pCold-MBP-(Ent-TEV)<br/></div><div>Enzyme: NcoI/ XhoI&nbsp;&nbsp;&nbsp; antibiotics<br/></div><div></div><br />
4/10/15<br />
<br />
[[File:2434.JPG|RTENOTITLE]]<br />
The 1021 digestion product is about 1200bp, is longer than expected insert (900bp).<br />
The Transformation plate don't have any clonies.&nbsp;<br />
<br />
'''<span style="font-size:larger">4/20/15&nbsp; repeat the subclone again.</span>'''<br />
<br />
Clone 2367 was used as the vector (2367 was used&nbsp; as vector).<br />
<br />
[[File:2418 2434 clone.JPG|371x176px|RTENOTITLE]]<br />
<br />
Gel purification, digestion, ligation.<br />
<br />
<span style="font-size:larger">'''Screening'''</span>:<br />
<br />
[[File:2418 2434 screening.JPG|RTENOTITLE]]<br />
<br />
There wasn't positive colony. The vector size also change,which could be caused by a mutation on the vector and make a cutting size. need to repeat again, this time use the 2185 and 2367 both as vector to compare.<br />
<br />
[[File:2434 2436 screening 5-1-15.JPG|RTENOTITLE]]<br />
<br />
== '''Conclusion''' ==<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
<br />
== ==<br />
<br />
&nbsp;<br />
[[Category:Pages with broken file links]] [[Category:Cloning]]</div>Excellgen