https://eln.excellgen.com/index.php?action=history&feed=atom&title=2437-pT7A30-Tat-NLS-Cas9 2026-10-18T05:16:58Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2437-pT7A30-Tat-NLS-Cas9&diff=14361&oldid=prev 2015-05-05T18:15:05Z

<p></p> <p><b>New page</b></p><div>&amp;nbsp;&#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; &#039;&#039;&#039;2015-April&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 2437&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;By: Xin Xu&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Results&#039;&#039;&#039;:&amp;nbsp;<br /> <br /> Clone TAT into vector 2332 pT7A30-Cas9 by NdeI +NcoI<br /> <br /> PCR 1708 using<br /> <br /> PCR on 1708 pT7A3-HTNCre&lt;br/&gt;[[File:2437 PCR.JPG|201x139px|RTENOTITLE]]<br /> <br /> Digested the vector 2332 pT7A30-Cas9 by NdeI +NcoI&lt;br/&gt;[[File:2437 vector.jpg|RTENOTITLE]]<br /> <br /> Ligation, transformation, screening:<br /> <br /> [[File:2437 screening.JPG|RTENOTITLE]] &amp;nbsp;<br /> <br /> PCR screening:<br /> <br /> The primers used for screening was inside the gene: 2437-Tat-NLS-Nco1-R;2437-Tat-NLS-Nde1-F<br /> <br /> All the clonies have the right PCR Product.<br /> <br /> <br /> <br /> PCR screening again, using one primer was on the vector Pet-seq-F2 and one inside the gene 2437-Tat-NLS-Nco1-R<br /> <br /> <br /> <br /> [[File:2437 PCR SCREENING with pet.JPG|230x138px|RTENOTITLE]]<br /> <br /> The colonies have a faint product. Also the -ve control also have the product. contaminated?<br /> <br /> sent No3 to sequence.</div>

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