https://eln.excellgen.com/index.php?action=history&feed=atom&title=2461_2462_2467_Protein_expression_screening 2026-10-18T04:04:36Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2461_2462_2467_Protein_expression_screening&diff=14572&oldid=prev 2015-07-06T16:31:18Z

<p></p> <p><b>New page</b></p><div>[[File:2461 2462 2467 copy.jpg]]<br /> <br /> <br /> <br /> {| border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; height=&quot;272&quot; width=&quot;847&quot;<br /> |-<br /> | Lane&lt;br/&gt;<br /> | Name&lt;br/&gt;<br /> | Protein Name&lt;br/&gt;<br /> | Clone ID&lt;br/&gt;<br /> | Host&lt;br/&gt;<br /> | Induction&lt;br/&gt;<br /> | Purification&lt;br/&gt;<br /> | pI/Mw&lt;br/&gt;<br /> |-<br /> | 1&lt;br/&gt;<br /> | <br /> pET28b-6H-Tat-NLS-Cas9<br /> <br /> <br /> <br /> | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&lt;br/&gt;<br /> | 2461&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG&amp;nbsp; RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | 9.36 / 164777.95&lt;br/&gt;<br /> |-<br /> | 2&lt;br/&gt;<br /> | pT7A3E-Tn5-ChiBD-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2462&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG&amp;nbsp; RT o/n&lt;br/&gt;<br /> | Ni Beads&lt;br/&gt;<br /> | &lt;font face=&quot;Cambria, serif&quot;&gt;&lt;font style=&quot;font-size: 11pt&quot; size=&quot;2&quot;&gt;&lt;span style=&quot;text-decoration: none&quot;&gt;8.41 / 83.8&lt;/span&gt;&lt;/font&gt;&lt;/font&gt;&lt;br/&gt;<br /> |-<br /> | 3&lt;br/&gt;<br /> | pET30-30GFP-Cas9&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2467&lt;br/&gt;<br /> | <br /> &amp;nbsp; 5 ml,&lt;br/&gt;Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | <br /> &lt;font face=&quot;Cambria, serif&quot;&gt;&lt;font style=&quot;font-size: 11pt&quot; size=&quot;2&quot;&gt;&lt;span style=&quot;text-decoration: none&quot;&gt;7.66 / 189.5&lt;/span&gt;&lt;/font&gt;&lt;/font&gt;<br /> <br /> |-<br /> | 4&lt;br/&gt;<br /> | Marker&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}<br /> <br /> Weak band in 2462 pT7A3E-Tn5-ChiBD-6H(83.8kDa)<br /> <br /> no expression in 2467&amp;nbsp;pET30-30GFP-Cas9 (189.5 kDa)and 2461 pET28b-6H-Tat-NLS-Cas9(166.9 kDa)<br /> <br /> <br /> <br /> [[File:2461 2462.jpg|RTENOTITLE]]<br /> <br /> {| border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot; width=&quot;832&quot;<br /> |-<br /> | Lane&lt;br/&gt;<br /> | Name&lt;br/&gt;<br /> | Protein Name&lt;br/&gt;<br /> | Clone ID&lt;br/&gt;<br /> | Host&lt;br/&gt;<br /> | Induction&lt;br/&gt;<br /> | Purification&lt;br/&gt;<br /> | pI/Mw&lt;br/&gt;<br /> |-<br /> | 1&lt;br/&gt;<br /> | <br /> pET28b-6H-Tat-NLS-Cas9<br /> <br /> <br /> <br /> | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&lt;br/&gt;<br /> | 2461&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG&amp;nbsp; RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | 9.36 / 164777.95&lt;br/&gt;<br /> |-<br /> | 2&lt;br/&gt;<br /> | pET28b-6H-Tat-NLS-Cas9&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2461&lt;br/&gt;<br /> | <br /> 5 ml,<br /> <br /> Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG&amp;nbsp; RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | 9.38 / 166.9&lt;br/&gt;<br /> |-<br /> | 3&lt;br/&gt;<br /> | Marker&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | <br /> <br /> <br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |-<br /> | 4&lt;br/&gt;<br /> | pT7A3E-Tn5-ChiBD-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2462&lt;br/&gt;<br /> | <br /> &amp;nbsp; 5 ml,&lt;br/&gt;Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | <br /> &lt;font face=&quot;Cambria, serif&quot;&gt;&lt;font style=&quot;font-size: 11pt&quot; size=&quot;2&quot;&gt;&lt;span style=&quot;text-decoration: none&quot;&gt;8.41 / 83.8&lt;/span&gt;&lt;/font&gt;&lt;/font&gt;<br /> <br /> |-<br /> | 5&lt;br/&gt;<br /> | pT7A3E-Tn5-ChiBD-6H&lt;br/&gt;<br /> | &lt;br/&gt;<br /> | 2462&lt;br/&gt;<br /> | <br /> &amp;nbsp;5 ml,&lt;br/&gt;Nico21(DE3)plysN<br /> <br /> | 0.2 mM IPTG RT o/n&lt;br/&gt;<br /> | Ni beads&lt;br/&gt;<br /> | <br /> &lt;font face=&quot;Cambria, serif&quot;&gt;&lt;font style=&quot;font-size: 11pt&quot; size=&quot;2&quot;&gt;&lt;span style=&quot;text-decoration: none&quot;&gt;8.41 / 83.8&lt;/span&gt;&lt;/font&gt;&lt;/font&gt;<br /> <br /> |}<br /> [[Category:Protein Purification]]</div>

Paul.xu