https://eln.excellgen.com/index.php?action=history&feed=atom&title=2467-pET30-30GFP-Cas9 2026-10-18T18:04:09Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2467-pET30-30GFP-Cas9&diff=14466&oldid=prev 2015-05-18T21:22:35Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of &lt;span dir=&quot;auto&quot;&gt;2467-pET30-30GFP-Cas9&lt;/span&gt;&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Subcloning of Cas9 gene from 2332&amp;nbsp;pT7A30-Cas9 into 2436 pET30-30GFP<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2015-May<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Xin<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039;: ==<br /> <br /> Digest the vector and insert plasmid by NcoI/XhoI<br /> <br /> &#039;&#039;&#039;[[File:2467 digestion.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> The Cas9 gene is about 4300bp. the total size of 2332 is 9557bp. So the bottom line of the insert is what we interested.<br /> <br /> Ligation and transformation. The transformation plate only have one colony growing. Mini pre this one clony and digest.<br /> <br /> == &#039;&#039;&#039;Screening&#039;&#039;&#039;: ==<br /> <br /> [[File:2467.JPG ]]<br /> <br /> <br /> <br /> &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039;<br /> <br /> <br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> {| cellspacing=&quot;1&quot;<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> <br /> <br /> <br /> {|<br /> |-<br /> | &lt;br/&gt;<br /> |}<br /> [[Category:Cloning]]</div>

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