https://eln.excellgen.com/index.php?action=history&feed=atom&title=2476_pEC2-TREC 2476 pEC2-TREC - Revision history 2026-10-18T10:26:40Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2476_pEC2-TREC&diff=15297&oldid=prev Excellgen at 19:04, 22 September 2016 2016-09-22T19:04:49Z <p></p> <p><b>New page</b></p><div>== &lt;span class=&quot;mw-headline&quot; id=&quot;Cloning_of_1251_pET28b-KOD-sso7d&quot;&gt;&#039;&#039;&#039;Cloning of 2476 pEC2-TREC&#039;&#039;&#039;&lt;/span&gt; ==<br /> <br /> PCR using blood genomic DNA as template<br /> <br /> [[File:Trec PCR 1.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> Digest with EcoR1 and Xho1, clone into EcoR1 and Xho1 digested pEC2.<br /> <br /> [[File:PEC2 digestion 1.JPG|210x275px|PEC2 digestion 1.JPG]]<br /> <br /> <br /> <br /> <br /> <br /> === &#039;&#039;&#039;&lt;span class=&quot;mw-headline&quot; id=&quot;Colony_Screening&quot;&gt;Colony Screening&lt;/span&gt;&#039;&#039;&#039; ===<br /> <br /> PCR screening<br /> <br /> [[File:Trec PCR screening 2.JPG|RTENOTITLE]]<br /> <br /> {| style=&quot;width: 226px&quot; border=&quot;1&quot; cellpadding=&quot;1&quot; cellspacing=&quot;1&quot;<br /> |-<br /> | style=&quot;width: 88px; text-align: center&quot; | Band1&lt;br/&gt;<br /> | style=&quot;width: 89px; text-align: center&quot; | Band2&lt;br/&gt;<br /> | style=&quot;width: 61px; text-align: center&quot; | Band13-18&lt;br/&gt;<br /> |-<br /> | style=&quot;width: 88px; text-align: center&quot; | No Template&lt;br/&gt;<br /> | style=&quot;width: 89px; text-align: center&quot; | Primer&lt;br/&gt;<br /> | style=&quot;width: 61px; text-align: center&quot; | 2476&lt;br/&gt;<br /> |}<br /> <br /> Make miniprep and digest with EcoR1 and Xho1.<br /> <br /> [[File:Trec digestion .JPG|RTENOTITLE]]<br /> [[Category:Cloning]]</div> Excellgen