https://eln.excellgen.com/index.php?action=history&feed=atom&title=2489_pMiniT-TREC-ACTB-ALB 2026-10-18T17:31:41Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2489_pMiniT-TREC-ACTB-ALB&diff=15398&oldid=prev 2016-09-30T18:50:24Z

<p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 2489 pMiniT-TREC-ACTB-ALB&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of &lt;span dir=&quot;auto&quot;&gt;2489 pMiniT-TREC-ACTB-ALB&lt;/span&gt;<br /> <br /> &#039;&#039;&#039;Date:&amp;nbsp;&#039;&#039;&#039; 2016-09-30<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 2489&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp; xxx<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> PCR of 2489<br /> <br /> Template: Trec-combo<br /> <br /> Primer: 2477-ALB-NdeI-F, 2478-betaActin-XhoI-R<br /> <br /> PCR cycle:<br /> <br /> 1 min at 94 oC<br /> <br /> 30 cycles:&lt;br/&gt;30 s at 94 oC&lt;br/&gt;30 s at 55 oC&lt;br/&gt;1.5 min at 72 oC<br /> <br /> 5 min at 72 oC<br /> <br /> [[File:2489 PCR.JPG|RTENOTITLE]]<br /> <br /> Make miniprep and digest with EcoRI.<br /> <br /> [[File:2489 digestion.JPG|RTENOTITLE]]<br /> <br /> PCR screening<br /> <br /> [[File:2489 PCR screening.JPG|RTENOTITLE]]<br /> <br /> Use Forward and reverse primer from Biolabs.<br /> <br /> Send sample 1 and 5 for sequencing. Sample 5 is the desired product.<br /> <br /> <br /> <br /> &amp;nbsp; &amp;nbsp;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> == ==<br /> [[Category:Cloning]]</div>

Crm