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2503 pCold-GST-VVTopo - Revision history
2026-10-18T15:05:59Z
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Crm at 19:35, 28 June 2018
2018-06-28T19:35:31Z
<p></p>
<p><b>New page</b></p><div>'''Subclone and Vector digestion'''<br />
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[[File:IMG 0860.JPG|RTENOTITLE]]<br />
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'''Miniprep digestion of 2503_3122_3197_3191'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| lane<br />
| Enzymes<br />
| Size<br />
| Note<br />
|-<br />
| 2503<br />
| 1-4<br />
| Nde1+Xho1<br />
| 951bp<br />
| #1,2 seems good<br />
|-<br />
| 3122<br />
| 5-8<br />
| Nde1+Xho1<br />
| 1743bp<br />
| #5,6,8 seems good<br />
|-<br />
| 3197<br />
| 9-12<br />
| BamH1+Nco1<br />
| 1 band<br />
| may not good<br />
|-<br />
| 3191<br />
| 13-16<br />
| BamH1+Nco1<br />
| 722bp<br />
| #14,15,16 seems good<br />
|}<br />
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[[File:IMG 0872.JPG|RTENOTITLE]]sequencing sample #:&nbsp; result: no priming, possible reason:&nbsp;??<br />
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need to do: digest with EcoR1+ Xho1, Pst1+ Xho1, and Nde1 + Xho1 5/31/2018:&nbsp;Sequencing sample #:&nbsp; result:&nbsp; &nbsp;vector<br />
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Date:6/5/2018==<br />
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'''2503 subclone'''<br />
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[[File:IMG 0928.JPG|RTENOTITLE]]<br />
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'''Miniprep of 3196_2503'''<br />
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[[File:IMG 090612.JPG|RTENOTITLE]]<br />
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'''2503 subclone'''<br />
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[[File:IMG 0928.JPG|RTENOTITLE]]<br />
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'''Miniprep of 2503_3210_931-1'''<br />
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digestion using Nhe1 + Xho1<br />
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vector is&nbsp;3161 pCold-GST--Myc-HA-Flag-V5<br />
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[[File:IMG 0943.JPG|RTENOTITLE]]<br />
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Subclone from 832 pMiniT-VVTopo&nbsp;<br />
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[[Category:Cloning]]</div>
Crm