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252-pLenti-IRES-TFR-EGFP - Revision history
2026-10-18T08:50:13Z
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Yingying.lu at 15:40, 7 February 2012
2012-02-07T15:40:50Z
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<p><b>New page</b></p><div>Cloning of pLenti-IRES-TFR-EGFP (252) <br />
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Purpose: to clone pLenti-IRES-TFR-EGFP by Gene Synthesis <br />
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Date: 2012-1-24 to 2012-1-27 <br />
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Notebook: Xiaojing Wang <br />
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Page: 004143---004144 <br />
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Clone ID: 252 <br />
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Experimenters: Xiaojing Wang <br />
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Results <br> <br />
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[[Image:252 PCR 1.24.2012 S.JPG]]<br> <br />
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digest:<br> <br />
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[[Image:252 digest 1.24.2012 S.JPG]][[Image:252's vector 1.24.2012 s.JPG]]<br> <br />
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digest PCR product and vector with Xho1 + Mlu1, run gel <br />
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the left band shows 252 insert, the right picture shows vector (pLentiPlus, 152) <br />
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gel purify, ligation, transformation <br />
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Colony Screening <br> <br />
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[[Image:252 digest 1.27.2012 s.JPG]]<br> <br />
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Select 5 clones to mini-prep, digested by Xho1 and Mlu1. 252-2,-3,-4,-5 may be correct. Control is 252-1. <br />
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Select 252-2 to sequence.<br> <br />
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[[Category:Cloning]]</div>
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