https://eln.excellgen.com/index.php?action=history&feed=atom&title=257-pET28b-HTNCre-ssfGFP 257-pET28b-HTNCre-ssfGFP - Revision history 2026-10-18T15:59:08Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=257-pET28b-HTNCre-ssfGFP&diff=3140&oldid=prev Ruili.su at 15:18, 24 January 2012 2012-01-24T15:18:45Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 257-pET28b-HTNCre-ssfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 257-pET28b-HTNCre-ssfGFP by PCR&lt;br&gt; <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-17-- <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:257&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:257 PCR 1-17-2012.JPG]]&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &#039;&#039;&#039;pTriEx-HTNC 298 as template&#039;&#039;&#039;&lt;br&gt; <br /> <br /> &#039;&#039;&#039;&amp;nbsp;6XHis-NcoI-F + Cre-Nhe1-R&amp;nbsp; &#039;&#039;&#039;&lt;br&gt; <br /> <br /> [[Image:257 clone 1-17-2012.JPG]]&lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 373px; height: 80px;&quot;<br /> |-<br /> | &lt;br&gt; <br /> | band 1&lt;br&gt; <br /> | band 2&lt;br&gt;<br /> |-<br /> | &lt;br&gt; <br /> | PCR product&lt;br&gt; <br /> | pET28b-ssfGFP 169&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt; <br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1+ Nhe1&lt;br&gt;<br /> |}<br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> === Subcloning ===<br /> <br /> Date <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1- <br /> <br /> Make miniprep and digest with <br /> <br /> &lt;br&gt; <br /> <br /> &lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *&lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt; <br /> <br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot;<br /> |-<br /> | &lt;br&gt;<br /> |}<br /> <br /> == &#039;&#039;&#039;Cloning of pET28b-HTNCre-ssfGFP&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of pET28b-HTNCre-ssfGFP <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2012-1-20 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:257&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; yingying <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;[[Image:468 vector +257 clone 1-20-2012.JPG]]&#039;&#039;&#039; <br /> <br /> {| cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; border=&quot;1&quot; style=&quot;width: 396px; height: 96px;&quot;<br /> |-<br /> | &lt;br&gt;<br /> | band 3&lt;br&gt;<br /> | &amp;nbsp;band 4&lt;br&gt;<br /> |-<br /> | &lt;br&gt;<br /> | PCR product&lt;br&gt;<br /> | pET28b-ssfGFP 169&lt;br&gt;<br /> |-<br /> | digest enzymes&lt;br&gt;<br /> | colspan=&quot;2&quot; | &amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp;&amp;nbsp; Nco1+ Nhe1&lt;br&gt;<br /> |}<br /> &lt;br&gt;<br /> <br /> === Subcloning ===<br /> <br /> <br /> <br /> &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2012-1-23&lt;br&gt; <br /> <br /> Make miniprep and digest with Nco1+ Nhe1&lt;br&gt; <br /> <br /> [[Image:257_digest_detect_1-24-1012.JPG]]<br /> <br /> &amp;nbsp;clone 1,2,3,4,5 may right&lt;br&gt; <br /> &lt;br&gt;<br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> &lt;br&gt;<br /> &lt;br&gt;<br /> === ===<br /> <br /> &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Ruili.su