Paul.xu at 21:23, 4 October 2019

2019-10-04T21:23:03Z

New page

[[File:2624 cloning.JPG|RTENOTITLE]][[File:2624 digestion.JPG|RTENOTITLE]]

expression screening

[[File:VLP expression screening 9 28 19.jpg|RTENOTITLE]]

{| style="width:500px" cellspacing="1" cellpadding="1" border="1"
|-
| Lane
| ID
| Name
| Induction
| Sample
| Expression
|-
| 1
| 971
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC
| S
| 
|-
| 2
| 971
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| p
| 
|-
| 3
| 2616
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| S
| 
|-
| 4
| 2616
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| P
| 
|-
| 5
| 2564
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| S
| 
|-
| 6
| 2621 
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| S
| 
|-
| 7
| ?
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| 
| 
|-
| 8
| 2621
| 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| S
| 
|-
| 9
| 2615
| pCold-MBP-(tev)-GST-(3c)-Ac-LysargiNase 
| 5052 o/n 37 oCm 1 mM 3 hours 37 oC 
| S
| 
|-
| M
| 
| 
| 
| 
| 
|-
| 10
| 2564
| 
| 5052 o/n 18 oCm 1 mM 12 hours 37 oC, cell lysed 
| P
| 
|-
| 11
| 2624
| pCold-NSP4-iFlp 
| 5052 o/n 18 oCm 1 mM 12 hours 37 oC, cell lysed 
| secreted to medium
| 
|-
| 12
| 2624
|  
| 5052 o/n 18 oCm 1 mM 12 hours 18 oC 
| S 
| 
|-
| 13
| 2624
|  
| 5052 o/n 18 oCm 1 mM 12 hours 18 oC 
| P
|  
|-
| 14
| 2624
| 
| 5052 o/n 18 oCm 1 mM 12 hours 18 oC 
| P, + SDS sample buffer
|  
|}

[[File:2624 expression screening.jpg|RTENOTITLE]]
[[Category:Cloning]]

2624 - Revision history

Paul.xu at 21:23, 4 October 2019