Paul.xu: Created page with "File:2625 2626 subcloning.JPGFile:2626 digetion.JPGFile:2626 digestion2.JPG Expression Screening File:2628 2626 2625 screening.jpg Expression host: Nico2..."

2019-10-09T20:43:31Z

Created page with "File:2625 2626 subcloning.JPG File:2626 digetion.JPG File:2626 digestion2.JPG Expression Screening File:2628 2626 2625 screening.jpg Expression host: Nico2..."

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[[File:2625 2626 subcloning.JPG]][[File:2626 digetion.JPG]][[File:2626 digestion2.JPG]]

Expression Screening

[[File:2628 2626 2625 screening.jpg]]
Expression host: Nico21(DE3)pLysN, [[Category:Cloning]]0.5 M IPTG
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| ID
| Name
| Sample
| Induction
| Expression
|-
| M
|  
| PageRuler Plus 
| 
| 
| 
|-
| 1
| 2628 
| pET28b-hFth-iFlp 
| from 0.5 ml of 8 L exp, 37 oC 5 hours, S
| 0.5 mM IPTG
| 
|-
| 2
| 2628 
| 
| from 15 ml small scale, 18 oC induction, S
| 1 mM
| 
|-
| 3
| 2628 
| 
| from 15 ml small scale, 18 oC induction, P
| 1
| 
|-
| 4
| 2628 
| 
| from 15 ml small scale,37 oC induction, S
| 1
| 
|-
| 5
| 2628 
| 
| from 15 ml small scale,37 oC induction, P
| 1
| 
|-
| 6
| 2626
| pCold-GST-CYFRA 21-1-avitag-6H 
| 15 ml small scale, 18 oC induction, S 
| 1
| 
|-
| 7
|
2626

+ 2625

|
pCold-GST-CYFRA 21-1-avitag-6H

+

pCold-GST-Ac-LysargiNase

|
from 2626 15 ml small scale, 18 oC induction, P 

+

from 2625 15 ml small scale, 18 oC induction, S 

| 1
| 
|-
| 8
| 2625
| pCold-GST-Ac-LysargiNase 
| 15 ml small scale, 18 oC induction, P 
| 1
| 
|-
| M
| 
| 
| 
| 
| 
|-
| 9
| 
| 
| 
| 
| 
|}

2626 - Revision history

Paul.xu: Created page with "File:2625 2626 subcloning.JPGFile:2626 digetion.JPGFile:2626 digestion2.JPG Expression Screening File:2628 2626 2625 screening.jpg Expression host: Nico2..."