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2640 - Revision history
2026-10-18T22:40:25Z
Revision history for this page on the wiki
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Paul.xu at 22:57, 1 November 2019
2019-11-01T22:57:33Z
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<p><b>New page</b></p><div>[[File:2640 subcloning.JPG|RTENOTITLE]][[File:2640 2632 digestion.JPG|RTENOTITLE]]<br />
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11/1/2019<br />
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Use Nico21(DE3)pRare2pLysS, 15 ml, induced with 1 mM IPTG, 37 oC 5 hours<br />
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cre in Nico21(DE3)pLysN, (from freezer, not freshly transformed) 8L, induce 37 oC 6 hours, 0.15% arabinose., use 50 ml Ni column<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| Lane<br />
| 1<br />
| <br/><br />
| MW<br />
| Expression<br />
|-<br />
| 1<br />
| 1078<br />
| pET28b-scIHF2, soluble<br/><br />
| 22.8<br />
| N<br />
|-<br />
| 2<br />
| 406<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pET28b-Int</span>, soluble<br/><br />
| 42.5<br />
| N<br />
|-<br />
| 3<br />
| 1185<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pDB.His.MBP-Int</span>, soluble<br/><br />
| 83.7<br />
| Y<br />
|-<br />
| 4<br />
| 2640<br />
| pCold-MBP-(Ent-TEV)-scIHF, soluble<br/><br />
| 66.9<br />
| N<br />
|-<br />
| 5<br />
| 2640<br />
| pCold-MBP-(Ent-TEV)-scIHF, inclusion body<br/><br />
| 66.9<br/><br />
| Y<br />
|-<br />
| 6~12<br />
| 1010&nbsp;<br />
| Cre,&nbsp;<br/><br />
| <br/><br />
| could be low<br />
|-<br />
| 13<br />
| 1078<br />
| pET28b-scIHF2<br/><br />
| 22.8<br/><br />
| N<br />
|-<br />
| 14<br />
| 406<br />
| <span style="color: rgb(0, 0, 0); font-family: Arial, Verdana, sans-serif; font-size: 12px; white-space: nowrap;">pET28b-Int</span><br/><br />
| 42.5<br/><br />
| N<br />
|-<br />
| M<br />
| <br/><br />
| PageRuler Plus<br />
| <br/><br />
| <br/><br />
|}<br />
<br />
[[File:1185 2640 expression.jpg|RTENOTITLE]]<br />
[[Category:Cloning]]</div>
Paul.xu