https://eln.excellgen.com/index.php?action=history&feed=atom&title=2763 2026-10-18T10:10:44Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2763&diff=20963&oldid=prev 2020-06-16T19:28:18Z

<p></p> <p><b>New page</b></p><div>[[File:2763 2773 PCR.JPG|RTENOTITLE]]<br /> <br /> [[File:2759 2763 2773 XhoI.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> [[File:2760 2763 2770 2773 PCR.JPG|RTENOTITLE]]<br /> <br /> PCR from genomic DNA using superhifi II enzyme mix<br /> <br /> 6/6/2020 [[File:2763 2772 2773 2779 PCR.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> PCR again from genomic DNA<br /> <br /> [[File:2763 2779 2770 PCR.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> <br /> <br /> [[File:2763 PCR NEB5a.JPG|RTENOTITLE]]<br /> <br /> [[File:2763 2773 pMiniT2 NdeI XhoI.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> 6/10/2020<br /> <br /> digestion miniprep DNA with Xho1<br /> <br /> [[File:2773 2763 digestion.JPG|RTENOTITLE]]<br /> <br /> subclone 2763-pMiniT2 to 2660-pET29 with NdeI+XhoI<br /> <br /> [[File:2763 pET29.JPG|RTENOTITLE]] [[File:2763 pminiT2 digestion.JPG|RTENOTITLE]]<br /> <br /> [[File:2763 gibson vector.JPG|RTENOTITLE]] <br /> <br /> 20200616 2763 pJet NdeI+XhoI in Cutsmart, 37oC, 1hour<br /> [[File:2746 2763 2773 2779 pJet digestion.JPG|RTENOTITLE]]<br /> [[Category:Cloning]]</div>

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