Paul.xu at 01:12, 29 June 2020

2020-06-29T01:12:37Z

New page

[[File:2746 2770 2779 PCR.JPG|RTENOTITLE]]

PCR from genomic DNA using superhifi II enzyme mix

6/6/2020 [[File:2763 2772 2773 2779 PCR.JPG|RTENOTITLE]]

PCR again from genomic DNA

[[File:2763 2779 2770 PCR.JPG|RTENOTITLE]]

[[File:2770 2779 PCR.JPG|RTENOTITLE]]

[[File:2773 2779 digestion.JPG|RTENOTITLE]]

[[File:2772 2746 2779 2778.JPG|RTENOTITLE]]

[[File:2779 pJet XhoI XbaI.JPG|RTENOTITLE]]

20200616 NdeI+HindIII, buffer2.1 ,37oC, 1hour

[[File:2746 2763 2773 2779 pJet digestion.JPG|RTENOTITLE]]

== 2779 gibson ==

20200617 to get the vector from #483 digest with NdeI+HindIII in Buffer 2, 37oC , 40min

[[File:2779 gibson vector.JPG|RTENOTITLE]]

== Protein Expression ==

[[File:2779|RTENOTITLE]][[File:2779 protein expression.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Lane
| Name
| Desc
|-
| 1
| N protein 2 ul
| 
|-
| 2
| N protein 5 ul
| 
|-
| 3
| S protein 5 ul
| BEI
|-
| 4
| RBD  5 ul
| BEI
|-
| 5
| 2779 Turner 37 oC 2 hours, 7.5 ml, 0.2% rhamnose
| 
|-
| 6
| 2779 NEB 37 oC 2 hours,  7.5 ml, 0.2% rhamnose
| 
|-
| 7
| 2746 1mM IPTG
| 
|-
| 8
| 2723
| 
|-
| 9
| 2779 NEB 25 oC o/n  7.5 ml, 0.2% rhamnose
| cell dead
|-
| 10
| 2779 NEB 25 oC o/n  7.5 ml, 0.2% rhamnose
| cell dead
|}
[[Category:Pages with broken file links]]

== http://18.206.161.119/eln/images/6/66/2779_protein_exp_screening2.jpg ==
[[Category:Pages with broken file links]] [[Category:Cloning]]

2779 - Revision history

Paul.xu at 01:12, 29 June 2020