https://eln.excellgen.com/index.php?action=history&feed=atom&title=284_pAAV-CMV-mkate2 284 pAAV-CMV-mkate2 - Revision history 2026-10-18T10:58:29Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=284_pAAV-CMV-mkate2&diff=1495&oldid=prev Hylei at 19:37, 10 August 2011 2011-08-10T19:37:53Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254)&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; SubCloning of CFP from pet28b-cfp to the pAAV-MCS (254). <br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2011-8-3 to 2011-8-10 <br /> <br /> &#039;&#039;&#039;Notebook:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Clone ID:&#039;&#039;&#039; <br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039; Robert, Mindy, Yolanda&lt;br&gt; <br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Subcloning ===<br /> <br /> Date: 2011-8-4 <br /> <br /> Digest&amp;nbsp; (clone #) with &lt;br&gt; <br /> <br /> Digest&amp;nbsp; (clone #) with &lt;br&gt; <br /> <br /> cut out insert and vector, ligate and transform &lt;br&gt; <br /> <br /> === Colony Screening ===<br /> <br /> Date: 2011-8-10&lt;br&gt; <br /> <br /> Make miniprep and digest with BamH1 + Xho1&lt;br&gt; <br /> <br /> [[Image:PAAV-mkate2_2011_8_10.jpg]]&lt;br&gt; <br /> <br /> &amp;nbsp;Lane 1: 1 kb ladder&lt;br&gt; <br /> <br /> &amp;nbsp;Lanes 2-6: pAAV-mkate2<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> *pAAV-mkate2 clones &lt;br&gt;<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> <br /> Restriction digestion &lt;br&gt; <br /> <br /> Shows&amp;nbsp; &lt;br&gt; <br /> <br /> [[Category:Cloning]]</div> Hylei