https://eln.excellgen.com/index.php?action=history&feed=atom&title=2889 2026-10-18T19:23:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2889&diff=21519&oldid=prev 2022-03-26T13:57:06Z

<p>Created page with &quot;T7-VEE-FLuc &lt;pre&gt;2889-F: TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA 2889-R: ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG (CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa) clone into pMiniT, then s...&quot;</p> <p><b>New page</b></p><div>T7-VEE-FLuc<br /> &lt;pre&gt;2889-F:<br /> TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA<br /> 2889-R:<br /> ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG<br /> (CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa)<br /> clone into pMiniT, then subclone using Nde1-Not1<br /> <br /> <br /> <br /> &gt;201228-13_O07_1F85DAA023.ab1 1192, 12/29/2020<br /> seq with pminiT-F<br /> NNNNNNNTNNNNNNNCGACCGATTGTTAGGTAATCGTCACCTGCAGGAAG<br /> GTTTAAACGCATTTAGGTGACACTATAGAAGTGTGTATCGCTCGAGGGAT<br /> CCGAATTCAGGAGGTAAAAACCATGATGCGGCCGCGGCCGGCTTACAATT<br /> TGGACTTTCCGCCCTTCTTGGCCTTTATGAGGATCTCTCTGATTTTTCTT<br /> GCGTCGAGTTTTCCGGTAAGACCTTTCGGTACTTCGTCCACAAACACAAC<br /> TCCTCCGCGCAACTTTTTCGCGGTTGTTACTTGACTGGCGACGTAATCCA<br /> CGATCTCTTTTTCCGTCATCGTCTTTCCGTGCTCCAAAACAACAACGGCG<br /> GCGGGAAGTTCACCGGCGTCATCGTCGGGAAGACCTGCCACGCCCGCGTC<br /> GAAGATGTTGGGGTGTTGTAACAATATCGATTCCAATTCAGCGGGGGCCA<br /> CCTGATATCCTTTGTATTTAATTAAAGACTTCAAGCGGTCAACTATGAAG<br /> AAGTGTTCGTCTTCGTCCCAGTAAGCTATGTCTCCAGAATGTAGCCATCC<br /> ATCCTTGTCAATCAAGGCGTTGGTCGCTTCCGGATTGTTTACATAACCGG<br /> ACATAATCATAGGTCCTCTGACACATAATTCGCCTCTCTGATTAACGCCC<br /> AGCGTTTTCCCGGTATCCAGATCCACAACCTTCGCTTCAAAAAATGGAAC<br /> AACTTTACCGACCGCGCCCGGTTTATCATCCCCCTCGGGTGTAATCAGAA<br /> TAGCTGATGTAGTCTCAGTGAGCCCATATCCTTGTCGTATCCCTGGAAGA<br /> TGGAAGCGTTTTGCAACCGCTTCCCCGACTTCTTTCGAAAGAGGTGCGCC<br /> CCCAGAAGCAATTTCGTGTAAATTAGATAAATCGTATTTGTCAATCAGAG<br /> TGCTTTTGGCGAAGAATGAAAATAGGGTTGGTACTAGCAACGCACTTTGA<br /> ATTTTGTAATCCTGAAGGGATCGTAAAAACAGCTCTTCTTCAAATCTATA<br /> CATTAAGACGACTCGAAATCCACATATCAAATATCCGAGTGTAGTAAACA<br /> TTCCAAAACCGTGATGGAATGGAACAACACTTAAAATCGCAGTATCCGGA<br /> ATGATTTGATTGCCAAAAATAGGATCTCTGGCATGCGAGAATCTGACGCA<br /> GGCAGTTCTATGCGGAAGGGCCNACCCTTAGGTAACCCAGTA<br /> <br /> rv<br /> TACTGGGTTACCTAAGGGTNGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA<br /> GCCGGCC<br /> Not1<br /> GCGGCCGCA<br /> TCATGGTTTTTACCTCCTGAATTCGGATCCCTCGAGCGATACACACTTCTATAGTGTCACCTAAATGCGTTTAAACCTTCCTGCAGGTGACGATTACCTAACAATCGGTCGNNNNNNNANNNNNNN<br /> Score Expect Identities Gaps Strand<br /> 1960 bits(1061) 0.0 1065/1067(99%) 1/1067(0%) Plus/Plus<br /> Query 1 TACTGGGTTACCTAAGGGT-NGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 59<br /> ||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||<br /> Sbjct 629 TACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCA 688<br /> <br /> Query 60 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 119<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 689 TGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGT 748<br /> <br /> Query 120 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 179<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 749 TCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCG 808<br /> <br /> Query 180 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 239<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 809 AGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAA 868<br /> <br /> Query 240 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 299<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 869 AATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGAT 928<br /> <br /> Query 300 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 359<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 929 TGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAA 988<br /> <br /> Query 360 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 419<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 989 AGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGG 1048<br /> <br /> Query 420 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 479<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1049 GCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGC 1108<br /> <br /> Query 480 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 539<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1109 GGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAAC 1168<br /> <br /> Query 540 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 599<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1169 GCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTA 1228<br /> <br /> Query 600 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 659<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1229 TGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGG 1288<br /> <br /> Query 660 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 719<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1289 AGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTT 1348<br /> <br /> Query 720 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 779<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1349 AATTAAATACAAAGGATATCAGGTGGCCCCCGCTGAATTGGAATCGATATTGTTACAACA 1408<br /> <br /> Query 780 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 839<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1409 CCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCC 1468<br /> <br /> Query 840 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 899<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1469 CGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTA 1528<br /> <br /> Query 900 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 959<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1529 CGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGA 1588<br /> <br /> Query 960 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1019<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1589 AGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAA 1648<br /> <br /> Query 1020 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1066<br /> |||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1649 GGCCAAGAAGGGCGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCA 1695<br /> <br /> <br /> &gt;210108-18_A05_1F85DAA019.ab1 1225, 1/9/2021<br /> 2889 in MiniT2 seq with pminiT-R primer<br /> <br /> no good missing TCATATGTA Nde1 site<br /> <br /> <br /> NNNNNNTNNNNNGTATTTAGAAATAAACAAATAGGGGTTCCGCCCGCGAA<br /> ATTAATACGACTCACTATAGGGAGACGCACAT<br /> Not1<br /> GCGGCCGC CTCGAGAATT<br /> CTGACGTCTTAATTAATTATTATC<br /> <br /> AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCG<br /> CTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA<br /> ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGA<br /> ATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGC<br /> TGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTC<br /> TTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGC<br /> GAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGC<br /> AGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC<br /> GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAA<br /> AACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATC<br /> TACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGT<br /> GACAAAACAATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACC<br /> TAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATG<br /> CCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA<br /> AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATA<br /> TTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGC<br /> TGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTA<br /> CCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGA<br /> TTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAG<br /> AAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACA<br /> AGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAG<br /> GGGGAAGATAAAACCGGGCGCGGGC<br /> <br /> Alignment statistics for match #1<br /> Score Expect Identities Gaps Strand<br /> 1989 bits(1077) 0.0 1089/1094(99%) 3/1094(0%) Plus/Plus<br /> Query 130 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 189<br /> |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 20 AGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGA 79<br /> <br /> Query 190 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 249<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 80 GGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGG 139<br /> <br /> Query 250 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 309<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 140 AACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA 199<br /> <br /> Query 310 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 369<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 200 AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAAT 259<br /> <br /> Query 370 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 429<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 260 CGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTAT 319<br /> <br /> Query 430 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 489<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 320 CGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTAT 379<br /> <br /> Query 490 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 549<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 380 GAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAA 439<br /> <br /> Query 550 CGTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 609<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 440 CGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA 499<br /> <br /> Query 610 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 669<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 500 CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGA 559<br /> <br /> Query 670 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 729<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 560 ATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTC 619<br /> <br /> Query 730 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 789<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 620 CTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAG 679<br /> <br /> Query 790 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 849<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 680 ATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTT 739<br /> <br /> Query 850 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 909<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 740 AAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG 799<br /> <br /> Query 910 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 969<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 800 TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCA 859<br /> <br /> Query 970 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 1029<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 860 GGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAG 919<br /> <br /> Query 1030 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 1089<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 920 CACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACC 979<br /> <br /> Query 1090 TCTTTCGAAAGAAGTCGGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1149<br /> |||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 980 TCTTTCGAAAGAAGTC-GGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGAC 1038<br /> <br /> Query 1150 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCCAAGGGGGAAGAT 1209<br /> ||||||||||||||||||||||||||||||||||||||||||||||| ||||||| |||<br /> Sbjct 1039 AAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCG-AGGGGGATGAT 1097<br /> <br /> Query 1210 AAAACCGGGCGCGG 1223<br /> ||| ||||||||||<br /> Sbjct 1098 AAA-CCGGGCGCGG 1110<br /> <br /> PCR again using<br /> <br /> 10 uM 2895-F3 TCTAGTCCGCCAAGTCTAGcatatgtaaGGCGCGCCCAC<br /> 1uL 2889-F: TCATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACA<br /> and<br /> 10 uM 2889-R: ttGCGGCCGCGGCCGGCTTACAATTTGGACTTTCCG<br /> (CGGAAAGTCCAAATTGTAAGCCGGCCGCGGCCGCaa)<br /> clone into pMiniT, then subclone using Nde1-Not1<br /> <br /> &gt;210119-09_E07_1F85DAA000.ab1 1347, 1/20/2021<br /> 2889 in pVEE good<br /> NNNNNNNNNNNTNNGCTACCTGATGGACTACGACATAGTCTAGTCCGCCA<br /> AGTCTAG<br /> Nde1<br /> CATATG TAAGGCGC<br /> Kozak<br /> GCCACCATGGAAGACGCCAAAAACATAAA<br /> GAAAGGCCCGGCGCCATTCTATCCTCTAGAGGATGGAACCGCTGGAGAGC<br /> AACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCT<br /> TTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGA<br /> AATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAA<br /> ATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCG<br /> GTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACAT<br /> TTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCG<br /> TAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAA<br /> AAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTA<br /> CCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCG<br /> GTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACA<br /> ATTGCACTGATAATGAATTCCTCTGGATCTACTGGGTTACCTAAGGGTGT<br /> GGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATC<br /> CTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTT<br /> CCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATG<br /> TGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTAC<br /> GATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTA<br /> TTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAA<br /> TTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGG<br /> AAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGG<br /> CTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAA<br /> ACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGGGGA<br /> TCTGGATACCGGGAAAAANCTGGGCGTTAATCAGAAGGGGAATTAGGNTC<br /> AAAAGACCTTGAATTAGTCCGGTTTGTAAACANTCCGGAAGNNCCACCCC<br /> TTGATGANAGGNNGGNGGGTNNNTNNGGGAAANNNNTTTANNGGAAA<br /> <br /> <br /> Score Expect Identities Gaps Strand<br /> 2178 bits(1179) 0.0 1221/1242(98%) 9/1242(0%) Plus/Plus<br /> CATATGTAAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 131<br /> |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> CATATG-AAGGCGCGCCACCATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCTCTAGAG 98<br /> <br /> Query 132 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 191<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 99 GATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGA 158<br /> <br /> Query 192 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 251<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 159 ACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAA 218<br /> <br /> Query 252 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 311<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 219 ATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATC 278<br /> <br /> Query 312 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 371<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 279 GTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATC 338<br /> <br /> Query 372 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 431<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 339 GGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATG 398<br /> <br /> Query 432 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 491<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 399 AACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAAC 458<br /> <br /> Query 492 GTGCaaaaaaaaTTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 551<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 459 GTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTAC 518<br /> <br /> Query 552 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 611<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 519 CAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAA 578<br /> <br /> Query 612 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 671<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 579 TACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAATTCC 638<br /> <br /> Query 672 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 731<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 639 TCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGA 698<br /> <br /> Query 732 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 791<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 699 TTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTA 758<br /> <br /> Query 792 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 851<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 759 AGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGT 818<br /> <br /> Query 852 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 911<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 819 GGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAG 878<br /> <br /> Query 912 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 971<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 879 GATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGC 938<br /> <br /> Query 972 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 1031<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 939 ACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCT 998<br /> <br /> Query 1032 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1091<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 999 CTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAA 1058<br /> <br /> Query 1092 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1151<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1059 GGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAA 1118<br /> <br /> Query 1152 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTGG-GGATCTGGATACC 1210<br /> |||||||||||||||||||||||||||||||||||||||||||| | |||||||||||||<br /> Sbjct 1119 CCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACC 1178<br /> <br /> Query 1211 GGGAAAAANCTGGGCGTTAATCAGA-AGGGGAATTA-GGNTCAAAAGACCT-TGAATTA- 1266<br /> ||||||| |||||||||||||||| ||| |||||| | ||| | ||||| ||| |||<br /> Sbjct 1179 GGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGA-TTAT 1237<br /> <br /> Query 1267 GTCCGGTT-TGTAAACANTCCGGAAGNN-CCACC-CCTTGAT 1305<br /> |||||||| |||||||| |||||||| ||| | |||||||<br /> Sbjct 1238 GTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGAT 1279<br /> <br /> <br /> Range 2: 1 to 38GraphicsNext MatchPrevious MatchFirst Match<br /> Alignment statistics for match #2<br /> Score Expect Identities Gaps Strand<br /> 65.8 bits(35) 4e-14 38/39(97%) 1/39(2%) Plus/Plus<br /> Query 58 96<br /> |||||| ||||||||||||||||||||||||||||||||<br /> Sbjct 1 38<br /> &lt;/pre&gt;</div>

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