https://eln.excellgen.com/index.php?action=history&feed=atom&title=2939 2026-10-18T08:00:27Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2939&diff=21521&oldid=prev 2022-03-26T19:27:40Z

<p></p> <p><b>New page</b></p><div>&lt;pre&gt;not working---&gt;<br /> PCR from 2931<br /> Gibson mix cloning<br /> <br /> 2935-F4<br /> TCTAGTCCGCCAAGTCTAGcatatgCTAGCtagagaataaac <br /> <br /> 1uM 2935-F1<br /> atcatatgCTAGCtagagaataaactagtattcttctggtccccacagactc<br /> 1 uM 2938-F1<br /> cttctggtccccacagactcagagagaaccGCCACCATGGACTATAAGGAC<br /> 2939-R1<br /> CTATGTAAGCAGCTTGCGGCCGCTATTCCTTTTTCTTTTTTGCCTG<br /> (CAGGCAAAAAAGAAAAAGGAATAGCGGCCGCAAGCTGCTTACATAG)<br /> <br /> ========<br /> Use Nde1-AflII for cloning into 2895 or 2926<br /> 2939-R2<br /> TGTAAGCAGCTTaAGCTATTCCTTTTTCTTTTTTGCCTGG<br /> (CCAGGCAAAAAAGAAAAAGGAATAGCTtAAGCTGCTTACA)<br /> <br /> ===<br /> Enzyme-free cloning<br /> <br /> &lt;s&gt;1uM 2935-F1<br /> atcatatgCTAGCtagagaataaactagtattcttctggtccccacagactc&lt;/s&gt;<br /> <br /> <br /> <br /> <br /> &lt;s&gt;EFC-F CatatgaagctTAATACGACTCACTATAagGagaataaactagtattcttc &lt;/s&gt;<br /> <br /> <br /> PCR insert from 2931<br /> EFC-F1 CatatgaagctTAATACGACTCACTATAagGagaataaac <br /> EFC- F2 TCACTATAagGagaataaac tagtattcttctggtcccca<br /> <br /> 1 uM 2938-F1<br /> cttctggtccccacagactcagagagaaccGCCACCATGGACTATAAGGAC<br /> <br /> <br /> <br /> <br /> 2939-EFC-R caCTAGTCCCCATCCTCGAGCTATTCCTTTTTCTTTTTTG<br /> (CAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGtg)<br /> <br /> <br /> PCR from vector 2893<br /> <br /> <br /> EFC-V-F CTCGAGGATGGGGACTAGtg<br /> EFC-V-R (2942-R)<br /> TGAGTCGTATTAagcttcatatGAGCTCGCGTTGCTGGCG<br /> (CGCCAGCAACGCGAGCTCatatgaagctTAATACGACTCA) <br /> <br /> <br /> digest with DpnI, purify, Gibson mix and transform 10G<br /> <br /> <br /> &lt;/pre&gt;<br /> <br /> <br /> <br /> [[File:2935 2938 2939 2942.JPG|RTENOTITLE]] T7ag-pA-eCas9<br /> &lt;pre&gt;Nde1 HindIII<br /> catatgaagct <br /> ( T7 ag)<br /> TAATACGACTCACTATA agG agaataaactagtattcttc tggtccccacagactcagagagaacc<br /> GCCACCATGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGGCCGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTGCGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGGCGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGGAA<br /> <br /> <br /> TAG<br /> <br /> Xho1<br /> CTCGAGGATGGGGAC<br /> TAGtgA<br /> <br /> GCCGGCC<br /> Not1<br /> GCGGCCGC<br /> <br /> GCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATT<br /> AAGCTGCTTACATAGAACTCGCGGCGATTG<br /> <br /> <br /> <br /> <br /> Sph1<br /> GCATGC<br /> <br /> <br /> CGCCTTAAAATTTTTATTTTATTTTTtCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaattaa<br /> <br /> Xba1<br /> tctaga<br /> acgcgTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTC <br /> <br /> <br /> <br /> <br /> &gt;210316-33_A11_1FC0DAA038.ab1 1225 3/17/21<br /> 2939 seq using VEE-seq-R<br /> NNNNNNNNNNNNNNGNNGCTTNTGNNTAAATGTTTTTTATTAGGCAGAAT<br /> CCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTT<br /> AGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCGC<br /> GGCCGCGGCCGGCTCACTAGTCCCCATCCTCGAGCTATTCCTTTTTCTTT<br /> TTTGCCTGGCCGGCCTTTTTCGTGGCCGCCGGCCTTTTGTCGCCTCCCAG<br /> CTGAGACAGGTCGATCCGTGTCTCGTACAGGCCGGTGATGCTCTGGTGGA<br /> TCAGGGTGGCGTCCAGCACCTCTTTGGTGCTGGTGTACCTCTTCCGGTCG<br /> ATGGTGGTGTCAAAGTACTTGAAGGCGGCAGGGGCTCCCAGATTGGTCAG<br /> GGTAAACAGGTGGATGATATTCTCGGCCTGCTCTCTGATGGGCTTATCCC<br /> GGTGCTTGTTGTAGGCGGACAGCACTTTGTCCAGATTAGCGTCGGCCAGG<br /> ATCACTCTCTTGGAGAACTCGCTGATCTGCTCGATGATCTCGTCCAGGTA<br /> GTGCTTGTGCTGTTCCACAAACAGCTGTTTCTGCTCATTATCCTCGGGGG<br /> AGCCCTTCAGCTTCTCATAGTGGCTGGCCAGGTACAGGAAGTTCACATAT<br /> TTGGAGGGCAGGGCCAGTTCGTTTCCCTTCTGCAGTTCGCCGGCAGAGGC<br /> CAGCATTCTCTTCCGGCCGTTTTCCAGCTCGAACAGGGAGTACTTAGGCA<br /> GCTTGATGATCAGGTCCTTTTTCACTTCTTTGTAGCCCTTGGCTTCCAGA<br /> AAGTCGATGGGATTCTTCTCGAAGCTGCTTCTTTCCATGATGGTGATCCC<br /> CAGCAGCTCTTTCACACTCTTCAGTTTCTTGGACTTGCCCTTTTCCACTT<br /> TGGCCACCACCAGCACAGAATAGGCCACGGTGGGGCTGTCGAAGCCGCCG<br /> TACTTCTTAGGGTCCCAGTCCTTCTTTCTGGCGATCAGCTTATCGCTGTT<br /> CCTCTTGGGCAGGATAGACTCTTTGCTGAAGCCGCCTGTCTGCACCTCGG<br /> TCTTTTTCACGATATTCACTTGGGGCATGCTCAGCACTTTCCGCACGGTG<br /> GCAAAATCCCGGCCCTTATCCCACACGATCTCCCCGGTTTCGCCGTTTGT<br /> CTCGATCAGAGGCGCCTTCCGGATCTCGCCGTTGGCCAGGGTAATCTCGG<br /> TCTTGAAAAAGTTCATGAAGTTGCC<br /> <br /> rev comp<br /> GGCAACTTCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGGCGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTANNCANAAGCNNCNNNNNNNNNNNNNN<br /> <br /> <br /> Score Expect Identities Gaps Strand<br /> 2207 bits(1195) 0.0 1197/1198(99%) 0/1198(0%) Plus/Plus<br /> Query 2 GCAACTTCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGG 61<br /> ||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3302 GCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGG 3361<br /> <br /> Query 62 CGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATT 121<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3362 CGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATT 3421<br /> <br /> Query 122 TTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGG 181<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3422 TTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGG 3481<br /> <br /> Query 182 TGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGA 241<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3482 TGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGA 3541<br /> <br /> Query 242 TCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGG 301<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3542 TCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGG 3601<br /> <br /> Query 302 CCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTG 361<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3602 CCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTG 3661<br /> <br /> Query 362 TGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCG 421<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3662 TGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCG 3721<br /> <br /> Query 422 ACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTA 481<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3722 ACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTA 3781<br /> <br /> Query 482 AGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAAC 541<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3782 AGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAAC 3841<br /> <br /> Query 542 TGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCA 601<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3842 TGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCA 3901<br /> <br /> Query 602 GCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGG 661<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3902 GCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGG 3961<br /> <br /> Query 662 AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAG 721<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 3962 AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAG 4021<br /> <br /> Query 722 TGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATA 781<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4022 TGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATA 4081<br /> <br /> Query 782 AGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAG 841<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4082 AGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAG 4141<br /> <br /> Query 842 CCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCA 901<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4142 CCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCA 4201<br /> <br /> Query 902 AAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGA 961<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4202 AAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGA 4261<br /> <br /> Query 962 TCGACCTGTCTCAGCTGGGAGGCGACaaaaggccggcggccacgaaaaaggccggccagg 1021<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4262 TCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGG 4321<br /> <br /> Query 1022 caaaaaagaaaaaGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTC 1081<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4322 CAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTC 4381<br /> <br /> Query 1082 GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAA 1141<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4382 GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAA 4441<br /> <br /> Query 1142 CTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTA 1199<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 4442 CTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTA 4499<br /> <br /> &gt;210316-33_G11_1FC0DAA039.ab1 1303 3/17/21<br /> 2939 seq using EFC-F1 CatatgaagctTAATACGACTCACTATAagGagaataaac <br /> good<br /> <br /> NNNNTNNNNNNNNNNNACTAGTATTCTTCTGGTCCCCACAGACTCAGAGA<br /> GAACCGCCACCATGGACTATAaGGACCACGACGGAGACTACAAGGATCAT<br /> GATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCG<br /> GAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCG<br /> GCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAG<br /> TACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCA<br /> CAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAA<br /> CAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGA<br /> CGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGC<br /> CAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGG<br /> AAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGAC<br /> GAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAA<br /> ACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCC<br /> TGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTG<br /> AACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGAC<br /> CTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACG<br /> CCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAAT<br /> CTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCT<br /> GATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACC<br /> TGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGAC<br /> CTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCT<br /> GGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAAAG<br /> TGAACACCGAGATCACCAGGGCCCCCCTGAACGCCTCTATGATCAGNNAT<br /> ACCAACAAGCACCACCAGGACCTGACCCTGCTGAAAGNNCTCGNGCGGAA<br /> ACNNNTGCCTGAGAANNNCAANNGATTTTCTTNCANNNAANNAGAAACGG<br /> CNACCCCGGTTNCTTNNNNNGNGGNCCNNCCNGAAAAATTCTCNNTNNNN<br /> TGC<br /> <br /> Score Expect Identities Gaps Strand<br /> 2132 bits(1154) 0.0 1182/1200(99%) 2/1200(0%) Plus/Plus<br /> Query 17 ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCGCCACCATGGACTATAaGGAC 76<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||<br /> Sbjct 22 ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCGCCACCATGGACTATAAGGAC 81<br /> <br /> Query 77 CACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCC 136<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 82 CACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCC 141<br /> <br /> Query 137 CCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGC 196<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 142 CCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGC 201<br /> <br /> Query 197 ATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAG 256<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 202 ATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAG 261<br /> <br /> Query 257 GTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAAC 316<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 262 GTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAAC 321<br /> <br /> Query 317 CTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGA 376<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 322 CTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGA 381<br /> <br /> Query 377 ACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTC 436<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 382 ACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTC 441<br /> <br /> Query 437 AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTG 496<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 442 AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTG 501<br /> <br /> Query 497 GTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTG 556<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 502 GTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTG 561<br /> <br /> Query 557 GCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACC 616<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 562 GCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACC 621<br /> <br /> Query 617 GACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGC 676<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 622 GACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGC 681<br /> <br /> Query 677 CACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATC 736<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 682 CACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATC 741<br /> <br /> Query 737 CAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTG 796<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 742 CAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTG 801<br /> <br /> Query 797 GACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATC 856<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 802 GACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATC 861<br /> <br /> Query 857 GCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTG 916<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 862 GCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTG 921<br /> <br /> Query 917 GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTG 976<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 922 GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTG 981<br /> <br /> Query 977 AGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC 1036<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 982 AGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC 1041<br /> <br /> Query 1037 GCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTG 1096<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 1042 GCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTG 1101<br /> <br /> Query 1097 AAAGTGAACACCGAGATCACCAGGGCCCCCCTGAACGCCTCTATGATCA-GNNATACCAA 1155<br /> | |||||||||||||||||||| ||||||||||| |||||||||||||| | |||| |<br /> Sbjct 1102 AGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACG-A 1160<br /> <br /> Query 1156 CAAGCACCACCAGGACCTGACCCTGCTGAAAGNNCTCGNGCGGAAACNNNTGCCTGAGAA 1215<br /> | |||||||||||||||||||||||||||||| |||| |||| | | ||||||||||<br /> Sbjct 1161 CGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAA 1220<br /> <br /> &lt;/pre&gt;<br /> <br /> [[Category:Cloning]]</div>

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