https://eln.excellgen.com/index.php?action=history&feed=atom&title=293f_cell_culture293f cell culture - Revision history2026-10-18T03:29:59ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=293f_cell_culture&diff=610&oldid=prevPaul.xu at 15:14, 6 April 20112011-04-06T15:14:03Z<p></p>
<p><b>New page</b></p><div>'''Plate''' for NTC DH5alpha <br />
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10g tryptone <br />
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5 g yeast extract <br />
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15 g agar QS to 880 mL with H2O <br />
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Autoclave 20 min 121°C, when cooled to ~50°C, add 120 mL 50% Sucrose (wt./vol.), mix, pour plates. <br />
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'''Liquid media''' <br />
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10g tryptone <br />
<br />
5 g yeast extract QS to 880 mL with H2O <br />
<br />
Autoclave 20 min 121°C <br />
<br />
When cooled to ~50°C, add 120 mL 50% Sucrose (wt./vol.), allow to cool before use. <br />
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[[Category:Buffer_Recipe]]</div>Paul.xu