https://eln.excellgen.com/index.php?action=history&feed=atom&title=2959 2026-10-18T08:00:22Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=2959&diff=21537&oldid=prev 2022-04-02T19:20:58Z

<p></p> <p><b>New page</b></p><div>T7AG-Flp<br /> <br /> subclone from 2895 to 2953 using Nde1-Mlu1<br /> <br /> [[File:2959 2960 digestion.JPG|RTENOTITLE]]<br /> <br /> TAATACGACTCACTATAAGGtcttctggtccccacagactcagagagaacccatatgtaaGGCGCGCCCACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAATTGCCACCATGGCCCCCAAGAAAAAGCGGAAAGTGATGAGCCAGTTCGACATCCTGTGCAAGACCCCCCCCAAGGTGCTCGTGCGGCAGTTCGTGGAAAGATTCGAGAGGCCCAGCGGCGAGAAGATCGCCTCTTGTGCCGCCGAGCTGACCTACCTGTGCTGGATGATCACCCACAACGGCACCGCCATCAAGCGGGCCACCTTCATGAGCTACAACACCATCATCAGCAACAGCCTGAGCTTCGACATCGTGAACAAGAGCCTGCAGTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCTGATCCCCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACAGCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCACAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGTACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCCTCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCCGGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGAGAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGCTGAAGGACAACCTCGTGCGGTCCTACAACAAGGCCCTGAAGAAAAACGCCCCCTACCCCATCTTCGCCATTAAGAACGGCCCCAAGTCCCACATCGGCCGGCACCTGATGACCAGCTTTCTGAGCATGAAGGGCCTGACAGAGCTGACCAACGTCGTGGGCAATTGGAGCGACAAGAGGGCCTCTGCCGTGGCCAGAACCACCTACACCCACCAGATCACAGCCATCCCCGACCACTACTTCGCCCTGGTGTCTCGGTACTACGCCTACGACCCCATCAGCAAAGAGATGATCGCCCTGAAGGACGAGACAAACCCCATCGAGGAATGGCAGCACATCGAGCAGCTGAAGGGCAGCGCCGAGGGCAGCATCAGATACCCTGCCTGGAACGGCATCATCTCCCAGGAAGTGCTGGACTACCTGAGCAGCTACATCAACCGGCGGATCTAGCGGCCGCTTAAGCTGCTTACCGCAAGCTGCTTACATAGAACTCGCGGCGATTGGCATGCCCGCCTTAAAATTTTTATTTTATTTTTtCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaattaatctagaacgcgTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTC<br /> &lt;pre&gt;&gt;A220401-011_M22_1FC0DAA006_premix.ab1 1231 4/2/2022<br /> 2959 T7AG-Flp<br /> sequencing using T7AG<br /> <br /> <br /> GNNNNNNNNANNCNNNTNNNNGATGAGACNTCTTNANATATTATTGTCAGAGCTCCCTGTAGGTGCTTTAGCGGGACGCTATGTCCGGATTCTGATGTAC<br /> CTGCTGAGGTAATATAGCCTTTTGTGGGAGATAATTGAGTTACATGTGCGGGATGAGATGCTGCCCTCGGCCCTGCCCTTCCGGGCTCCTTCNCTGCCAT<br /> TCCTCCATGGTCTTTGTCTCGCTGATCTTCGACATCATGTATTTGATGATGGGGTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAGGGTCGGGAA<br /> TGGNTGGATCCTGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCGATATCGTGTCCAGCCTGCAGCTGCAGTTC<br /> GAGAGCAGCGAGGAAGCCCACGAGGGCAACAGCCACAGCAAGAAGATGCT<br /> GAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCACAGAGAAGA<br /> TCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTG<br /> TACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNA<br /> CATCAAGAACGTGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACC<br /> TGGGCGTGATCATTCAGTGNCTCGTGACCGANACATAGACCAGCGTGTCC<br /> CGGCNCATCTACTTTTTCAGCGCCAGANGNCGGATCGACCCCCTGGTGTA<br /> CCTGGNCNAGTTCCTGAGAACAGCGAGCCCGTGCTGAANAGAGTGAACCG<br /> GACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC<br /> NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAACGCCCCACTN<br /> ACNCNTCTTCGCCATTAANANGGCCCAAGTCCNNACATCGGCGGCACTGA<br /> TGACCAGNTTTCNNAGCATGANNGNTGANNAGCTGACNNCGTCNTGGGTC<br /> AATTGNNTCNACANANNNNTNTNNNTNNNNNANNNTNNNNNNCNNATNNN<br /> NGCATCNCGACNNCTACTTNNCNTGNGNNNCNGNACTACNCNACGACCNT<br /> NNGNNNNNNNGATNNNNNAANNNNANNNANNNNNTNNGGATGNAGCNANT<br /> NGNNNNTNANNNNGNCNNNNGNATNNNNNNNNCNNNNGNNNNNNNNNNNT<br /> NTNNNTNNNNNNCNNNNNANNNNNCNNNNNN<br /> <br /> Alignment statistics for match #1<br /> Score Expect Identities Gaps Strand<br /> 920 bits(498) 0.0 642/726(88%) 15/726(2%) Plus/Plus<br /> Query 254 GTTCAAGGACAAGACCCAAAACGCCACCATCCTGNAAG-GGTCGGGAATG-GNTGGATCC 311<br /> ||||||| |||||||||| || |||||||||||| ||| | ||| | | |||||<br /> Sbjct 383 GTTCAAGTACAAGACCCAGAAGGCCACCATCCTGGAAGCCAGCCTGAAGAAGCT-GATCC 441<br /> <br /> Query 312 TGGCGTGGGAGTTTGCCTTCGTCCCCTACAACGGCCCTATTGTCCAGAGCGTTGTCACCG 371<br /> || ||||||||| || || ||||||||||||||| | |||||||| ||||||<br /> Sbjct 442 CCGCCTGGGAGTTTACCATCATCCCCTACAACGGCCAGAAGCACCAGAGCGACATCACCG 501<br /> <br /> Query 372 ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCCACGAGGGCAACA 431<br /> ||||||||||||||||||||||||||||||||||||||||||||||| || |||||||||<br /> Sbjct 502 ATATCGTGTCCAGCCTGCAGCTGCAGTTCGAGAGCAGCGAGGAAGCCGACAAGGGCAACA 561<br /> <br /> Query 432 GCCACAGCAAGAAGATGCTGAATGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGATATCA 491<br /> |||||||||||||||||||||| |||||||||||||||||||||||||||||||| ||||<br /> Sbjct 562 GCCACAGCAAGAAGATGCTGAAGGCCCTGCTGAGCGAGGGCGAGAGCATCTGGGAGATCA 621<br /> <br /> Query 492 CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT 551<br /> ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||<br /> Sbjct 622 CAGAGAAGATCCTGAACAGCTTCGAGTACACCAGCCGGTTCACCAAGACCAAGACCCTGT 681<br /> <br /> Query 552 ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCNACATCAAGAACG 611<br /> ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||<br /> Sbjct 682 ACCAGTTCCTGTTCCTGGCCACCTTTATCAACTGCGGCCGGTTCTCCGACATCAAGAACG 741<br /> <br /> Query 612 TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGNC 671<br /> |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |<br /> Sbjct 742 TGGACCCCAAGAGCTTCAAGCTGGTGCAGAACAAGTACCTGGGCGTGATCATTCAGTGCC 801<br /> <br /> Query 672 TCGTGACCGANACATAGACCAGCGTGTCCCGGCNCATCTACTTTTTCAGCGCCAGANGNC 731<br /> |||||||||| ||| |||||||||||||||||| |||||||||||||||||||||| | |<br /> Sbjct 802 TCGTGACCGAGACAAAGACCAGCGTGTCCCGGCACATCTACTTTTTCAGCGCCAGAGGCC 861<br /> <br /> Query 732 GGATCGACCCCCTGGTGTACCTGGNCNAGTTCCTGAG-AACAGCGAGCCCGTGCTGAANA 790<br /> |||||||||||||||||||||||| | |||||||||| |||||||||||||||||||| |<br /> Sbjct 862 GGATCGACCCCCTGGTGTACCTGGACGAGTTCCTGAGAAACAGCGAGCCCGTGCTGAAGA 921<br /> <br /> Query 791 GAGTGAACCGGACCGGCAACAGCANCTGCNACAAGCANGANTACCAGCTGCNTGANGGAC 850<br /> |||||||||||||||||||||||| || | ||||||| || |||||||||| ||| ||||<br /> Sbjct 922 GAGTGAACCGGACCGGCAACAGCAGCTCCAACAAGCAGGAATACCAGCTGC-TGAAGGAC 980<br /> <br /> Query 851 NATCTCNTGNNGGTCTNTACANNATGTCTCNTGANNAAAA-CGCCCCACTNACNCNTCTT 909<br /> | ||| || |||| |||| | | | | ||| |||| |||||| || | | ||||<br /> Sbjct 981 AACCTCGTGC-GGTCC-TACAACAAGGCCC-TGAAGAAAAACGCCCC-CTACCCCATCTT 1036<br /> <br /> Query 910 CGCCATTAANAN-GGCCC-AAGTCCNNACATCGGC-GGCA-CTGATGACCAGNTTTCNNA 965<br /> ||||||||| | ||||| |||||| |||||||| |||| ||||||||||| |||| |<br /> Sbjct 1037 CGCCATTAAGAACGGCCCCAAGTCCC-ACATCGGCCGGCACCTGATGACCAGCTTTCTGA 1095<br /> <br /> Query 966 GCATGA 971<br /> ||||||<br /> Sbjct 1096 GCATGA 1101<br /> <br /> &lt;/pre&gt;<br /> [[Category:Cloning]]</div>

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