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2966 - Revision history
2026-10-18T08:00:46Z
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Paul.xu: Paul.xu moved page 2956 to 2966
2022-05-15T00:49:36Z
<p>Paul.xu moved page <a href="/index.php?title=2956" class="mw-redirect" title="2956">2956</a> to <a href="/index.php?title=2966" title="2966">2966</a></p>
<p><b>New page</b></p><div>WO2010099296 Hyperactive PiggyBac transposases<br />
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catatgaagctTAATACGACTCACTATAagGATTAAT<br />
<br />
pfizer 5'UTR<br />
<br />
gagaataaactagtattcttctggtccccacagactcagagagaaccc<br />
<br />
Kozak<br />
<br />
gccacc<br />
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ATGGGCTCTAGCCTGGACGACGAGCACATCCTGAGCGCCCTGCTGCAGAGCGACGACGAACTGGTGGGCGAGGACAGCGACAGCGAGATCAGCGACCACGTGTCCGAGGACGACGTGCAGTCCGACACCGAGGAAGCCTTCATCGACGAGGTGCACGAAGTGCAGCCTACCAGCAGCGGCTCCGAGATCCTGGACGAGCAGAACGTGATCGAGCAGCCTGGCAGCTCCCTGGCCAGCAACAGAATCCTGACCCTGCCCCAGAGAACCATCAGAGGCAAGAACAAGCACTGCTGGTCCACCTCCAAGAGCACCAGGCGGAGCAGAGTGTCCGCCCTGAACATCGTGCGGAGCCAGAGGGGCCCCACCAGAATGTGCAGAAACATCTACGACCCCCTGCTGTGCTTCAAGCTGTTCTTCACCGACGAGATCATCAGCGAGATCGTGAAGTGGACCAACGCCGAGATCAGCCTGAAGAGGCGGGAGAGCATGACCGGCGCCACCTTCAGAGACACCAACGAGGACGAGATCTACGCCTTCTTCGGCATCCTGGTGATGACCGCCGTGAGAAAGGACAACCACATGAGCACCGACGACCTGTTCGACAGATCCCTGAGCATGGTGTACGTGTCCGTGATGAGCAGAGACAGATTCGACTTCCTGATCAGATGCCTGAGAATGGACGACAAGAGCATCAGACCCACCCTGCGGGAGAACGACGTGTTCACCCCCGTGCGGAAGATCTGGGACCTGTTCATCCACCAGTGCATCCAGAACTACACCCCTGGCGCCCACCTGACCATCGATGAGCAGCTGCTGGGCTTCAGAGGCAGATGCCCCTTCAGAATGTACATCCCCAACAAGCCCAGCAAGTACGGCATCAAGATCCTGATGATGTGCGACAGCGGCACCAAGTACATGATCAAC<br />
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change GGCATGC to GGaATGC to remove Sph1<br />
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GGaATGC<br />
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<br />
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CCTACCTGGGCAGAGGCACCCAGACAAACGGCGTGCCCCTGGGCGAGTACTACGTGAAAGAACTGAGCAAGCCTGTGCATGGCAGCTGCAGGAACATCACCTGCGACAACTGGTTCACCAGCATCCCCCTGGCCAAGAACCTGCTGCAGGAACCCTACAAGCTGACCATCGTGGGCACCGTGCGGAGCAACAAGCGGGAGATCCCAGAGGTGCTGAAGAACAGCAGATCCAGACCTGTGGGAACAAGCATGTTCTGCTTCGACGGCCCCCTGACCCTGGTGTCCTACAAGCCCAAGCCCGCCAAGATGGTGTACCTGCTGTCCAGCTGCGACGAGGACGCCAGCATCAACGAGAGCACCGGCAAGCCCCAGATGGTGATGTACTACAACCAGACCAAGGGCGGCGTGGACACCCTGGACCAGATGTGCAGCGTGATGACCTGCAGCAGAAAGACCAACAGATGGCCCATGGCCCTGCTGTACGGCATGATCAATATCGCCTGCATCAACAGCTTCATCATCTACAGCCACAACGTGTCCAGCAAGGGCGAGAAGGTGCAGAGCCGGAAGAAATTCATGCGGAACCTGTACATGAGCCTGACCTCCAGCTTCATGAGAAAGAGACTGGAAGCCCCCACCCTGAAGAGATACCTGCGGGACAACATCAGCAACATCCTGCCCAACGAAGTGCCAGGAACAAGCGACGACAGCACCGAGGAACCCGTGATGAAGAAGAGGACCTACTGCACCTACTGTCCCAGCAAGATCAGAAGAAAGGCCAACGCCAGCTGCAAGAAATGCAAAAAAGTGATCTGCCGGGAGCACAACATCGACATGTGCCAGAGCTGTTTC<br />
<pre>Stop</pre><br />
<br />
TGA<br />
<pre>TGA<br />
3’ UTR <br />
Xho1 Not1<br />
CTCGAG CGGCCGC<br />
change C to g to remove Sph1 site<br />
CTGGTACTGCATGgACGCAATGCTAGCT GCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGC TAGTTCCAGACACCTCC<br />
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CAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTT AGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCAC<br />
<br />
ACCCTGGAGCTAGCA <br />
</pre><br />
<pre /><br />
Sph1<br />
<br />
GCATGC<br />
<br />
====<br />
<br />
sequence for gene synthesis<br />
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CATATGAAGCTTAATACGACTCACTATAAGGATTAATGAGAATAAACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCCGCCACCATGGGCTCTAGCCTGGACGACGAGCACATCCTGAGCGCCCTGCTGCAGAGCGACGACGAACTGGTGGGCGAGGACAGCGACAGCGAGATCAGCGACCACGTGTCCGAGGACGACGTGCAGTCCGACACCGAGGAAGCCTTCATCGACGAGGTGCACGAAGTGCAGCCTACCAGCAGCGGCTCCGAGATCCTGGACGAGCAGAACGTGATCGAGCAGCCTGGCAGCTCCCTGGCCAGCAACAGAATCCTGACCCTGCCCCAGAGAACCATCAGAGGCAAGAACAAGCACTGCTGGTCCACCTCCAAGAGCACCAGGCGGAGCAGAGTGTCCGCCCTGAACATCGTGCGGAGCCAGAGGGGCCCCACCAGAATGTGCAGAAACATCTACGACCCCCTGCTGTGCTTCAAGCTGTTCTTCACCGACGAGATCATCAGCGAGATCGTGAAGTGGACCAACGCCGAGATCAGCCTGAAGAGGCGGGAGAGCATGACCGGCGCCACCTTCAGAGACACCAACGAGGACGAGATCTACGCCTTCTTCGGCATCCTGGTGATGACCGCCGTGAGAAAGGACAACCACATGAGCACCGACGACCTGTTCGACAGATCCCTGAGCATGGTGTACGTGTCCGTGATGAGCAGAGACAGATTCGACTTCCTGATCAGATGCCTGAGAATGGACGACAAGAGCATCAGACCCACCCTGCGGGAGAACGACGTGTTCACCCCCGTGCGGAAGATCTGGGACCTGTTCATCCACCAGTGCATCCAGAACTACACCCCTGGCGCCCACCTGACCATCGATGAGCAGCTGCTGGGCTTCAGAGGCAGATGCCCCTTCAGAATGTACATCCCCAACAAGCCCAGCAAGTACGGCATCAAGATCCTGATGATGTGCGACAGCGGCACCAAGTACATGATCAACGGAATGCCCTACCTGGGCAGAGGCACCCAGACAAACGGCGTGCCCCTGGGCGAGTACTACGTGAAAGAACTGAGCAAGCCTGTGCATGGCAGCTGCAGGAACATCACCTGCGACAACTGGTTCACCAGCATCCCCCTGGCCAAGAACCTGCTGCAGGAACCCTACAAGCTGACCATCGTGGGCACCGTGCGGAGCAACAAGCGGGAGATCCCAGAGGTGCTGAAGAACAGCAGATCCAGACCTGTGGGAACAAGCATGTTCTGCTTCGACGGCCCCCTGACCCTGGTGTCCTACAAGCCCAAGCCCGCCAAGATGGTGTACCTGCTGTCCAGCTGCGACGAGGACGCCAGCATCAACGAGAGCACCGGCAAGCCCCAGATGGTGATGTACTACAACCAGACCAAGGGCGGCGTGGACACCCTGGACCAGATGTGCAGCGTGATGACCTGCAGCAGAAAGACCAACAGATGGCCCATGGCCCTGCTGTACGGCATGATCAATATCGCCTGCATCAACAGCTTCATCATCTACAGCCACAACGTGTCCAGCAAGGGCGAGAAGGTGCAGAGCCGGAAGAAATTCATGCGGAACCTGTACATGAGCCTGACCTCCAGCTTCATGAGAAAGAGACTGGAAGCCCCCACCCTGAAGAGATACCTGCGGGACAACATCAGCAACATCCTGCCCAACGAAGTGCCAGGAACAAGCGACGACAGCACCGAGGAACCCGTGATGAAGAAGAGGACCTACTGCACCTACTGTCCCAGCAAGATCAGAAGAAAGGCCAACGCCAGCTGCAAGAAATGCAAAAAAGTGATCTGCCGGGAGCACAACATCGACATGTGCCAGAGCTGTTTCTGATGACTCGAGCGGCCGCCTGGTACTGCATGGACGCAATGCTAGCTGCCCCTTTCCCGTCCTGGGTACCCCGAGTCTCCCCCGACCTCGGGTCCCAGGTATGCTCCCACCTCCACCTGCCCCACTCACCACCTCTGCTAGTTCCAGACACCTCCCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACACCCTGGAGCTAGCAGCATGC<br />
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MGSSLDDEHILSALLQSDDELVGEDSDSEISDHVSEDDVQSDTEEAFIDEVHEVQPTSSGSEILDEQNVIEQPGSSLASNRILTLPQRTIRGKNKHCWSTSKSTRRSRVSALNIVRSQRGPTRMCRNIYDPLLCFKLFFTDEIISEIVKWTNAEISLKRRESMTGATFRDTNEDEIYAFFGILVMTAVRKDNHMSTDDLFDRSLSMVYVSVMSRDRFDFLIRCLRMDDKSIRPTLRENDVFTPVRKIWDLFIHQCIQNYTPGAHLTIDEQLLGFRGRCPFRMYIPNKPSKYGIKILMMCDSGTKYMINGMPYLGRGTQTNGVPLGEYYVKELSKPVHGSCRNITCDNWFTSIPLAKNLLQEPYKLTIVGTVRSNKREIPEVLKNSRSRPVGTSMFCFDGPLTLVSYKPKPAKMVYLLSSCDEDASINESTGKPQMVMYYNQTKGGVDTLDQMCSVMTCSRKTNRWPMALLYGMINIACINSFIIYSHNVSSKGEKVQSRKKFMRNLYMSLTSSFMRKRLEAPTLKRYLRDNISNILPNEVPGTSDDSTEEPVMKKRTYCTYCPSKIRRKANASCKKCKKVICREHNIDMCQSCF<br />
<br />
genewiz optimized <br />
<br />
ATGGGCTCTTCACTGGATGACGAGCACATCTTGTCGGCACTGCTGCAGAGCGATGACGAA CTGGTGGGAGAGGACAGCGACAGTGAGATATCTGACCACGTGTCTGAGGATGATGTGCAG TCTGACACCGAGGAGGCGTTCATTGACGAAGTCCATGAGGTGCAACCGACCTCCTCCGGT TCGGAGATCCTGGACGAACAGAACGTGATTGAGCAACCAGGCTCCTCTCTCGCTAGCAAT CGAATCCTCACCCTGCCGCAGCGGACCATCCGCGGAAAAAACAAGCACTGTTGGTCAACG TCCAAGTCTACGCGGCGCTCGAGGGTCTCGGCACTAAACATAGTACGATCCCAGCGCGGG CCCACTAGGATGTGCCGCAACATCTACGATCCGTTGCTGTGCTTCAAACTATTTTTCACC GACGAGATCATCTCTGAGATCGTGAAATGGACGAACGCCGAGATTTCTCTGAAGCGCCGC GAGAGTATGACCGGCGCCACGTTCAGGGATACCAACGAGGACGAGATCTACGCCTTCTTC GGAATCCTGGTCATGACAGCCGTGCGCAAGGACAACCATATGTCGACGGATGACCTTTTT GACCGCTCTCTGTCCATGGTGTACGTGTCTGTGATGTCGCGGGACCGCTTCGACTTCCTG ATCCGCTGCCTGCGCATGGATGACAAGTCGATCCGCCCCACACTCAGAGAGAACGACGTG TTCACCCCGGTGCGAAAGATCTGGGACTTGTTCATCCACCAGTGTATCCAGAACTACACC CCTGGCGCGCACCTGACCATTGATGAGCAGCTGCTCGGCTTCCGTGGCCGTTGTCCCTTC CGCATGTATATTCCCAACAAGCCATCGAAGTACGGCATTAAGATCCTGATGATGTGCGAC AGCGGTACCAAGTACATGATCAACGGTATGCCCTATCTGGGGCGGGGCACCCAGACCAAC GGCGTTCCGCTTGGGGAGTACTACGTGAAGGAGCTGTCCAAGCCCGTGCATGGTTCCTGC CGAAACATCACCTGTGACAACTGGTTTACCTCTATCCCTCTGGCGAAGAACCTGCTCCAG GAGCCATACAAACTGACCATCGTTGGTACCGTGAGGTCCAATAAGCGCGAGATCCCGGAG GTTCTGAAGAATTCCCGCTCCCGCCCCGTGGGGACTTCCATGTTCTGCTTTGACGGCCCT TTGACCCTTGTGAGCTACAAGCCCAAACCTGCCAAGATGGTCTACTTACTGAGTAGTTGC GACGAAGATGCTAGCATCAATGAGAGCACTGGCAAGCCTCAGATGGTGATGTATTACAAC CAGACTAAAGGCGGCGTCGACACTCTAGACCAGATGTGCTCTGTAATGACGTGCTCCCGC AAAACAAATCGTTGGCCCATGGCTCTCCTGTATGGAATGATCAATATCGCCTGTATCAAC TCCTTCATCATCTACTCCCACAACGTGTCCTCTAAGGGCGAGAAGGTCCAGTCCCGGAAG AAATTTATGCGTAATCTTTACATGTCACTTACCTCCAGCTTTATGAGAAAGCGCCTGGAG GCTCCTACCCTTAAGCGTTACTTGCGCGACAACATCAGCAACATCCTGCCTAACGAGGTA CCCGGGACTAGCGATGACTCTACTGAGGAGCCCGTCATGAAAAAACGTACTTACTGTACC TACTGCCCATCCAAGATTCGTCGTAAGGCCAACGCGTCCTGCAAGAAGTGCAAGAAGGTC ATCTGCCGCGAGCACAACATTGACATGTGCCAGAGCTGCTTC<br />
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MGSSLDDEHILSALLQSDDELVGEDSDSEISDHVSEDDVQSDTEEAFIDEVHEVQPTSSGSEILDEQNVIEQPGSSLASNRILTLPQRTIRGKNKHCWSTSKSTRRSRVSALNIVRSQRGPTRMCRNIYDPLLCFKLFFTDEIISEIVKWTNAEISLKRRESMTGATFRDTNEDEIYAFFGILVMTAVRKDNHMSTDDLFDRSLSMVYVSVMSRDRFDFLIRCLRMDDKSIRPTLRENDVFTPVRKIWDLFIHQCIQNYTPGAHLTIDEQLLGFRGRCPFRMYIPNKPSKYGIKILMMCDSGTKYMINGMPYLGRGTQTNGVPLGEYYVKELSKPVHGSCRNITCDNWFTSIPLAKNLLQEPYKLTIVGTVRSNKREIPEVLKNSRSRPVGTSMFCFDGPLTLVSYKPKPAKMVYLLSSCDEDASINESTGKPQMVMYYNQTKGGVDTLDQMCSVMTCSRKTNRWPMALLYGMINIACINSFIIYSHNVSSKGEKVQSRKKFMRNLYMSLTSSFMRKRLEAPTLKRYLRDNISNILPNEVPGTSDDSTEEPVMKKRTYCTYCPSKIRRKANASCKKCKKVICREHNIDMCQSCF<br />
[[Category:Cloning]]</div>
Paul.xu