https://eln.excellgen.com/index.php?action=history&feed=atom&title=2XFast_Ligase_Buffer2XFast Ligase Buffer - Revision history2026-10-18T03:27:03ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=2XFast_Ligase_Buffer&diff=1986&oldid=prevLuyingying at 22:14, 12 October 20112011-10-12T22:14:12Z<p></p>
<p><b>New page</b></p><div>2X Quick Ligation Reaction Buffer: '''for 4 ml buffer recipe '''<br> <br />
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132 mM Tris-HCL----- 528 ul autoclaved 1 M Tris-HCL (PH7.6)<br> <br />
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20 mM MgCl2 ----- 80 ul autoclaved 1 M MgCL<sub>2</sub> <br />
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2 mM dithiothreitol ----- 8 ul 1 M DTT (weight 0.154g DTT, dissolve with 1 ml autoclaved water, suggest that immediately store in the refrigerator after making this stock solution) <br />
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2 mM ATP ----- 800ul 10 mM ATP <br />
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15% Polyethylene glycol (PEG 6000) ----- 1.2 ml autoclaved 50% PEG 6000;&nbsp; <br />
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pH 7.6 @25°C&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; ----- at last, add 1384 ul autoclaved water to make the final 4 ml buffer.<br> <br />
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[[Category:Buffer_Recipe]]</div>Luyingying