Excellgen: Excellgen moved page 2X PCR buffer to 2X PCR buffer 6/30/2016

2016-07-16T19:11:08Z

Excellgen moved page 2X PCR buffer to 2X PCR buffer 6/30/2016

New page

PCR using--- as template, T7-F and T7-R as primers

{| border="1" cellpadding="1" cellspacing="1" width="373"
|-
| rowspan="5" style="width: 220px; text-align: center" colspan="1" | 2X buffer with 1.5 mM Mgcl2  final conc 
| style="width: 58px; text-align: center" | Control 
| style="width: 84px; text-align: center" | 2.5U NEB Taq 
|-
| style="width: 58px; text-align: center" | 1 
| style="width: 84px; text-align: center" | 0.5U Taq 
|-
| style="width: 58px; text-align: center" | 2 
| style="width: 84px; text-align: center" | 1U Taq 
|-
| style="width: 58px; text-align: center" | 3 
| style="width: 84px; text-align: center" | 2.5U Taq 
|-
| style="width: 58px; text-align: center" | 4 
| style="width: 84px; text-align: center" | 5U Taq 
|-
| rowspan="5" style="width: 220px; text-align: center" colspan="1" | 2X buffer with 2.5 mM Mgcl2 final conc 
| style="width: 58px; text-align: center" | Control 
| style="width: 84px; text-align: center" | 2.5U NEB Taq 
|-
| style="width: 58px; text-align: center" | 5 
| style="width: 84px; text-align: center" | 0.5U Taq 
|-
| style="width: 58px; text-align: center" | 6 
| style="width: 84px; text-align: center" | 1U Taq 
|-
| style="width: 58px; text-align: center" | 7 
| style="width: 84px; text-align: center" | 2.5U Taq 
|-
| style="width: 58px; text-align: center" | 8 
| style="width: 84px; text-align: center" | 5U Taq 
|}

20 cycle

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25 cycle

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30 cycle

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35 cycle

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6/30/2016

template 528 pLVX-IRES-EGFP， IRES-Xho1-F +  IRES-Mlu1-R， product 1.3 kb
<pre>ctcgag TACGTAAATTCCGCCCCTCTCCCTAACGTT

ACTGGCCGAA

GCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCCACCATATTGCCGTCTTTTGGCAATGTGAGGGCCCGGAAACCTGGCCCTGTCTTC
TTGACGAGCATTCCTAGGGGTCTTTCCCCTCTCGCCAAAGGAATGCAAGGTCTGTTGAATGTCGTGAAGGAAGCAGTTCCTC
TGGAAGCTTCTTGAAGACAAACAACGTCTGTAGCGACCCTTTGCAGGCAGCGGAACCCCCCACCTGGCGACAGGTGCCTCTG
CGGCCAAAAGCCACGTGTATAAGATACACCTGCAAAGGCGGCACAACCCCAGTGCCACGTTGTGAGTTGGATAGTTGTGGAA
AGAGTCAAATGGCTCTCCTCAAGCGTATTCAACAAGGGGCTGAAGGATGCCCAGAA
KpnI
GGTACC
CCATTGTATGGGATCTGATC
TGGGGCCTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGT
TTTCCTTTGAAAAACACGATGATAATATGGCCACAACC

EGFP
ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCAT
CCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAG
CTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGC
AGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCG
CACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATC
GAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCT
ATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCT
CGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCC
GCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCA
TGGACGAGCTGTACAAGTAA

ACGCGT</pre>

PCR primers
<pre>IRES-Xho1-F  2011_12_22    H10  ATCGCACTCGAGTACGTAAATTCCGCCCCTCTC
Xho1
atcgca ctcgag TACGTAAATTCCGCCCCTCTC</pre>

<pre>IRES-Mlu1-R 2011_12_22    H11     GATGCGTACGCGTTTACTTGTACAGCTCGTCCATG
(CATGGACGAGCTGTACAAGTAA ACGCGT acgcatc)
gatgcgtACGCGTTTACTTGTACAGCTCGTCCATG</pre>
[[Category:QC]]

2X PCR buffer 6/30/2016 - Revision history

Excellgen: Excellgen moved page 2X PCR buffer to 2X PCR buffer 6/30/2016