https://eln.excellgen.com/index.php?action=history&feed=atom&title=3010_pCMV5-Flag-kappa 2026-10-18T08:48:49Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3010_pCMV5-Flag-kappa&diff=17273&oldid=prev 2018-03-13T22:57:25Z

<p></p> <p><b>New page</b></p><div>[[File:3009 3035 3010 Xho1.JPG|RTENOTITLE]]<br /> <br /> http://www.excellgen.net/eln/images/2/2f/3001_3010_pMiniT2_digestion.JPG<br /> <br /> <br /> <br /> 2/4/2018<br /> <br /> 3010 pCMV5-Flag-kappa, Flag light&lt;br/&gt;need to fix from 3135-4-pMiniT2 &gt;as-F_E12.ab1 template&lt;br/&gt;it is actually 3010: missing&amp;nbsp;&lt;br/&gt;5&#039; end ATCATCGGATCCGCC&lt;br/&gt;3&#039; end: GGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACCATCATC&lt;br/&gt;using these oligos&lt;br/&gt;1 ul of 10 uM&lt;br/&gt;3010-Flag-VL-1C4ATCATCGGATCCGCCGCCACCATG&lt;br/&gt;1 ul of 1 uM&lt;br/&gt;3010-Flag-VL-2C5AGAGCAGCAGGAGCCCCAGGAGCTGAGCGGGGACCCTCATGTCCATGGTGGCGGCGGA&lt;br/&gt;1 ul of 1 uM&lt;br/&gt;3010-Flag-VL-11D2CGCTCCTTACACCTTTGGCGGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACC&lt;br/&gt;1 ul of 10 uM&lt;br/&gt;3010-Flag-VL-12D3GATGATGGTACCGCTAGCCCGC&lt;br/&gt;3135-4-pMiniT2 as template<br /> <br /> &lt;br/&gt;Vector Name: 2493 pCMV5-mIgG-kLC&lt;br/&gt;Restriction Enzymes: BamH1-Kpn1<br /> <br /> [[File:3001 3076 3010 3129 3130.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3010 miniprep:&#039;&#039;&#039;<br /> <br /> Restriction enzymes: BamHI+KpnI<br /> <br /> Time:15min<br /> <br /> Buffer:Fastdigest<br /> <br /> &#039;&#039;&#039;Result:good, sent to do sequencing.#4&#039;&#039;&#039;<br /> <br /> [[File:3010miniprep.JPG|RTENOTITLE]]<br /> [[Category:Pages with broken file links]] [[Category:Cloning]]</div>

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