https://eln.excellgen.com/index.php?action=history&feed=atom&title=3027_pCEH0-mIgG2b-HC3027 pCEH0-mIgG2b-HC - Revision history2026-10-18T10:11:22ZRevision history for this page on the wikiMediaWiki 1.44.0https://eln.excellgen.com/index.php?title=3027_pCEH0-mIgG2b-HC&diff=17290&oldid=prevCrm at 23:18, 13 March 20182018-03-13T23:18:20Z<p></p>
<p><b>New page</b></p><div><pre>3020-Xba1-Nhe1-F-> it is the same as IgG2b<br />
Xba1 Nhe1<br />
atcatc TCTAGA ttagaatc GCT AGC AGC GCT AAA ACG ACA CCC CCA TCT GTC TAT C<br />
A S S A K T T P P S V Y <br />
<br />
3027-Not1-Age1-R<br />
2b atcatcACCGGTGCGGCCGCTCATTTACCCGGAGACCGGGAG (CTCCCGGTCTCCGGGTAAATGA GCGGCCGC ACCGGT gatgat)<br />
S R S P G K -<br />
</pre><br />
<br />
[[File:Digestion of 3027 Ligation.JPG|RTENOTITLE]]<br />
<br />
'''Subclone of 3027:'''<br />
<br />
Vector:3017pCEH0<br />
<br />
Insert:3027pMiniT2<br />
<br />
restriction enzymes:MheI+NotI<br />
<br />
Time:30 min<br />
<br />
[[File:Subclone of 3027.JPG|RTENOTITLE]]<br />
<br />
'''Result''':So we cut out the band and did subclone<br />
<br />
3021:1-4 restriction enzymes:BssHII+XbaI<br />
<br />
3027:5-8 restrcition enzymes:NotI+NheI<br />
<br />
Buffer:Fastdigest<br />
<br />
Time:16min<br />
<br />
'''Result:'''seems good, but the result of sequencing is not,the product were actually are '''undigested vectors,3016pCEL0,3017pCEH0. so we collected them for other using.'''<br />
<br />
[[File:Miniprep Digestion 3021 3027.JPG|RTENOTITLE]]<br />
[[Category:Cloning]]</div>Crm