Paul.xu at 01:52, 27 December 2017

2017-12-27T01:52:07Z

New page

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[[File:3105 gene synthesis.JPG|RTENOTITLE]]

11/05/2017

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[[File:3105 3098 digestion.JPG|RTENOTITLE]]

3105 in pET28b

[[File:3098 3105.JPG|RTENOTITLE]]

in pET28b vector, digest with Nco1 -Xho1

good, combined 1, 2,3, 4

[[File:3098 3105 NcoI Xho1.JPG|RTENOTITLE]]--[[User:Paul.xu|Paul.xu]] ([[User_talk:Paul.xu|talk]]) 18:28, 21 November 2017 (EST)

== Protein Expression ==

[[File:Scan515.jpg|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width: 594px;"
|-
| Lane
| ID
| style="width: 134px;" | Protein Name
| style="width: 52px;" | MW, pI
| style="width: 44px;" | Tag
| style="width: 56px;" | Host
| style="width: 159px;" | Condition
| style="width: 78px;" | Expression
|-
| 1
| 927
| style="width: 134px;" | pET28b-Cid1 
| style="width: 52px;" | 42
| style="width: 44px;" | His
| style="width: 56px;" | Nico21(DE3), pLysN
| style="width: 159px;" | 15 ml, RM , 0.3M IPTG induce o/n
| style="width: 78px;" | Yes
|-
| 2
| 3097
| style="width: 134px;" | pET28b-pQbeta 
| style="width: 52px;" | 
| style="width: 44px;" | no
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, RM , 0.3M IPTG RM induce o/n 
| style="width: 78px;" | do not know
|-
| 3
| 3098
| style="width: 134px;" | pET28b-AP205 
| style="width: 52px;" | 
| style="width: 44px;" | no
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, RM , 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | do not know 
|-
| 4
| 3074
| style="width: 134px;" | pET28b-HA
| style="width: 52px;" | 
| style="width: 44px;" | no
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, RM , 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | do not know 
|-
| 5
| 3105
| style="width: 134px;" | pET28b-AaLS 
| style="width: 52px;" | 22
| style="width: 44px;" | His
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, RM , 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | Yes
|-
| 6
| M
| style="width: 134px;" rowspan="1" colspan="6" | 198, 98(Y), 62, 49, 38, 28, 17(Red), 14, 6, 3 
|-
| 7
| 2106
| style="width: 134px;" | pET28b-iFLP, KJE7
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His
| style="width: 56px;" | Tuner(DE3) 
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n
| style="width: 78px;" | 
|-
| 8
| 2115
| style="width: 134px;" | pT7A3-iFLP, KJE7 
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His 
| style="width: 56px;" | Tuner(DE3) 
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | 
|-
| 9
| 2106
| style="width: 134px;" | pET28b-iFLP, KJE8 
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His 
| style="width: 56px;" | Tuner(DE3)
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | 
|-
| 10
| 2115
| style="width: 134px;" | pT7A3-iFLP, KJE8 
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His 
| style="width: 56px;" | Tuner(DE3) 
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | 
|-
| 11
| 2106
| style="width: 134px;" | pET28b-iFLP,  pLysN 
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His 
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | 
|-
| 12
| 2115
| style="width: 134px;" | pT7A3-iFLP,  pLysN 
| style="width: 52px;" | 9.76 / 52.8 
| style="width: 44px;" | N-His 
| style="width: 56px;" | Nico21(DE3), pLysN 
| style="width: 159px;" | 15 ml, Rm, 0.3M IPTG  RM induce o/n 
| style="width: 78px;" | 
|}

[[File:See blue .jpg|RTENOTITLE]]

[[Category:Cloning]]

3105 pET28b-AaLS - Revision history

Paul.xu at 01:52, 27 December 2017