https://eln.excellgen.com/index.php?action=history&feed=atom&title=3152_pCEH0-Flag-VH-mIgG2a 2026-10-18T14:44:17Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3152_pCEH0-Flag-VH-mIgG2a&diff=17577&oldid=prev 2018-04-04T18:13:07Z

<p></p> <p><b>New page</b></p><div>Repeat subclone of 3152<br /> <br /> Restriction enzymes:BsrGI+NheI<br /> <br /> Buffer:NEB2<br /> <br /> Time:30min<br /> <br /> Result:Vector digest is good, but insert is not good.<br /> <br /> [[File:Repeat subclone of 3152.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep of 947_947pMiniT2_3131_3152&#039;&#039;&#039;<br /> <br /> 947:1-4 XbaI+NcoI-HF 1394 Cutsmart<br /> <br /> 947pMiniT2:5-8 ECoRI Cutsmart<br /> <br /> 3131:9-12 BssHII+XhoI 342 Cutsmart<br /> <br /> 3152:13-16 BsrGI+KpnI 371 NEB 2<br /> <br /> &#039;&#039;&#039;Result: sent lane 2, lane 16 to do sequence&#039;&#039;&#039;<br /> <br /> [[File:IMG 3621.JPG|RTENOTITLE]]<br /> <br /> == Date:4/2/2018 ==<br /> <br /> &#039;&#039;&#039;redo subclone of 3152&#039;&#039;&#039;<br /> <br /> Vector:3163<br /> <br /> Insert: 3009pMiniT<br /> <br /> enzymes:BsrGI+NheI<br /> <br /> [[File:IMG 0606.JPG|RTENOTITLE]]<br /> <br /> == Date:4/4/2018 ==<br /> <br /> &#039;&#039;&#039;miniprep digestion of 984_947_3152&#039;&#039;&#039;<br /> <br /> #Name Enzymes Size:<br /> <br /> 1-4:984 XbaI+NheI&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp;957bp<br /> <br /> 5&amp;nbsp;:982(973-1pMiniT) XbaI+NheI<br /> <br /> 6-9:947 XbaI+NcoI-HF&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; 1344bp<br /> <br /> 10:947pMiniT XbaI+NcoI-HF<br /> <br /> 11:947(from PCR cloning) XbaI+NcoI-HF<br /> <br /> 12-15:3152 BsrGI+NheI&amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; &amp;nbsp; 371bp<br /> <br /> 16: 3162(Vector) BsrGI+NheI<br /> <br /> [[File:IMG 0615.JPG|RTENOTITLE]]<br /> [[Category:Cloning]]</div>

Crm