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3166 pCMV-HA-VH-mIgG2b - Revision history
2026-10-18T11:51:47Z
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Paul.xu at 17:07, 30 May 2018
2018-05-30T17:07:50Z
<p></p>
<p><b>New page</b></p><div>Date:3/15/2018<br />
<br />
'''3166 PCR'''<br />
<br />
Primer:3166F+3166R<br />
<br />
Template:3017pCEL0<br />
<br />
buffer:Platinum<br />
<br />
length:60bp<br />
<br />
extension time:15s<br />
<br />
[[File:3166PCR.JPG|RTENOTITLE]]<br />
<br />
'''Date:3/21/2018'''<br />
<br />
'''Miniprep of 3166(from PCR)'''<br />
<br />
Restriction enzymes:BsrGI+NdeI<br />
<br />
Buffer:Fastdigest<br />
<br />
Time:30min<br />
<br />
'''Result:#1,#3,#5 have bands'''<br />
<br />
[[File:IMG 3638.JPG|RTENOTITLE]]<br />
<br />
'''Screening of 3166 miniprep'''<br />
<br />
1,3,5: BsrGI+SalI<br />
<br />
1'',3'',5'':BsrGI''<br />
<br />
Time:15min<br />
<br />
Buffer:Fastdigest<br />
<br />
'''Result:good'''<br />
<br />
[[File:IMG 3643.JPG|RTENOTITLE]]<br />
<br />
size is not right, should be 1.5 kB<br />
<br />
Date:2/26/2018<br />
<br />
'''Test of 947_945_3166_3165'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| Name<br />
| Enzymes<br />
| Size (bp)<br />
| Lane<br />
|-<br />
| 947<br />
| BglII<br />
| 646<br />
| 1,2,3<br />
|-<br />
| <br/><br />
| BssHII<br />
| 1856<br />
| 4,5,6<br />
|-<br />
| 945<br />
| BglII<br />
| 628<br />
| 11,12<br />
|-<br />
| <br/><br />
| BssHII<br />
| 1838<br />
| 13<br />
|-<br />
| 3165<br />
| SalI+BsrGI<br />
| 693<br />
| 8<br />
|-<br />
| <br/><br />
| HindIII+BsrGI<br />
| 441<br />
| 10<br />
|-<br />
| 3166<br />
| SalI+BsrGI<br />
| 1234<br />
| 9<br />
|-<br />
| <br/><br />
| XbaI<br />
| 2049<br />
| 7<br />
|-<br />
| <br/><br />
| BglII<br />
| 3259<br />
| to do<br />
|}<br />
<br />
[[File:IMG 36521.JPG|RTENOTITLE]]<br />
<br />
== Date:5/21/2018 ==<br />
<br />
'''Miniprep digestion of 1000_3200_3166'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Note<br />
|-<br />
| 1000<br />
| 1-3<br />
| HindIII+Xho1<br />
| 100<br />
| need to confirm the size<br />
|-<br />
| 3200<br />
| 4-6<br />
| BamH1+ECoR1<br />
| <br/><br />
| no insert band should be good,redo<br />
|-<br />
| 3166<br />
| 7-10<br />
| Sal1+BsrG1<br/><br />
| 1234<br />
| need to confirm the size<br />
|}<br />
<br />
[[File:Miniprep digestion of 1000 3200 3166.JPG|RTENOTITLE]]<br />
[[Category:Pages with broken file links]]sequencing using&nbsp;[[Category:Cloning]]mIgG2b-HC-PCR-R&nbsp; &nbsp; GACTCTGGGATGCCTGGTCAA</div>
Paul.xu