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3167 pCEH0-Myc-VH-mIgG2a - Revision history
2026-10-18T08:50:14Z
Revision history for this page on the wiki
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https://eln.excellgen.com/index.php?title=3167_pCEH0-Myc-VH-mIgG2a&diff=17350&oldid=prev
Crm at 17:29, 16 March 2018
2018-03-16T17:29:04Z
<p></p>
<p><b>New page</b></p><div>Date:3/12/2018<br />
<br />
'''subclone of 3167:'''<br />
<br />
Restriction enzymes:BsrGI+NheI<br />
<br />
Buffer:Fastdigest<br />
<br />
Time:15 min at 37oC<br />
<br />
'''Result: Vector is good, insert is not good, just cut out vector'''<br />
<br />
[[File:Subclone of 3167.JPG|RTENOTITLE]]<br />
<br />
Date:3/14/2018 '''Miniprep digestion of 3131_3161_3167'''<br />
<br />
Name length Enzymen Buffer<br />
<br />
3131:1-4 342 BssHII+XhoI Cutsmart<br />
<br />
3161:5-8 225 NdeI+XhoI Cutsmart<br />
<br />
3167:9-12 397 BsrGI+NheI NEB 2<br />
<br />
Time:40min<br />
<br />
[[File:Miniprepof 3131 3167 3161.JPG|RTENOTITLE]]<br />
<br />
'''Redo miniprep of 3131_3161_3167'''<br />
<br />
3131:1-4 BssHII+XhoI 342 Cutsmart<br />
<br />
3161:5-8 NdeI+XhoI 225 Cutsmart<br />
<br />
3167:9-12 BsrGI+NheI 397 NEB 2<br />
<br />
Time:40min<br />
<br />
[[File:Redo miniprep of 3131 3161 3167.JPG|RTENOTITLE]]<br />
<br />
'''Redo Subclone of 3167'''<br />
<br />
Vector:3163pCEH0<br />
<br />
Insert:3027pMiniT2<br />
<br />
BsrGI+KpnI<br />
<br />
Buffer:NEB 2<br />
<br />
Time:30min<br />
<br />
'''Result:Cut out and combine'''<br />
<br />
[[File:Redo Subclone 3167.JPG|RTENOTITLE]]<br />
<br />
== Date:3/16/2018 ==<br />
<br />
'''Miniprep of 945_3167'''<br />
<br />
945:1-4 Xba+NcoI Cutsmart 1326<br />
<br />
3167:5-8 BsrGI+NheI NEB 2 397<br />
<br />
Time:50min<br />
<br />
Result:<br />
<br />
[[File:Miniprepof 945 3167.JPG|RTENOTITLE]]<br />
[[Category:Cloning]]</div>
Crm