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3168 pCEH0-His-VH-mIgG2a - Revision history
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Crm at 18:48, 14 March 2018
2018-03-14T18:48:08Z
<p></p>
<p><b>New page</b></p><div>Subclone of 3168<br />
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Retsriction enzymes:BsrGI+NheI<br />
<br />
Time:30MIn<br />
<br />
Buffer:Fastdigest<br />
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Results:good, combine and do the step.<br />
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[[File:Subclone of new3168.JPG|RTENOTITLE]]<br />
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Miniprep of 3168_3132_3161<br />
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3168:1-4 BsrGI+NheI<br />
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3132:5-8 BsrGI+NheI<br />
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3161:9-12 NdeI+XhoI<br />
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Buffer:Fastdigest<br />
<br />
Time:30Min<br />
<br />
Result:#7 seems good, so sent to do sequencing, the rest of them are suspicious<br />
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[[File:Miniprep of 3168 3132 3161.JPG|RTENOTITLE]]<br />
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Date:3/14/2018<br />
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'''Miniprep digestion_3161_3168'''<br />
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3161:1,2 NdeI+XhoI Buffer:Cutsmart<br />
<br />
3168:3 BsrGI+NheI<br />
<br />
Buffer:NEB 2<br />
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Time:40min<br />
<br />
'''Result:seems good, do sequencing'''<br />
<br />
[[File:Miniprep of 3161 3168.JPG|RTENOTITLE]]<br />
[[Category:Cloning]]</div>
Crm