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3194 pCMV5-IL2-BCMA-(3c)-Fc-WPRE-OriP - Revision history
2026-10-18T05:56:40Z
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Paul.xu at 21:51, 18 June 2018
2018-06-18T21:51:04Z
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<p><b>New page</b></p><div>'''PCR of 3194'''<br />
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[[File:IMG 0818.JPG|RTENOTITLE]]<br />
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'''digestion of vector 3192_3194_3200'''<br />
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[[File:IMG 0823.JPG|RTENOTITLE]]<br />
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'''Miniprep digestion of 3122_984_3192_3194'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Enzymes<br />
| Size<br />
| Note<br />
|-<br />
| 3122<br />
| 1-4<br />
| Nde1+Xho1<br />
| <br/><br />
| <br/><br />
|-<br />
| 984<br />
| 5-8<br />
| Xba1+Nhe1<br />
| 957bp<br />
| Lane 8 maybe good,need to confirm the size<br />
|-<br />
| 3192<br />
| 9-12<br />
| Kpn1<br />
| <br/><br />
| need to confirm the size<br />
|-<br />
| 3194<br />
| 13-16<br />
| Nhe1+Kpn1<br />
| 174<br />
| Maybe vector<br />
|}<br />
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[[File:Miniprep dogestion of 3122 984 3192 3194.JPG|RTENOTITLE]]<br />
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not good, all&nbsp;could be parental vector PCR&nbsp; to verify: 3194-R1 GTACCCGCATTCGTTCCTTTCACTG + pCMV5-seq-F<br />
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== Date:6/5/2018 ==<br />
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'''redo''' '''PCR of 998_1000_3194'''<br />
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[[File:IMG 0908.JPG|RTENOTITLE]]<br />
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'''3233 subclone_3194 vector digestion'''<br />
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[[File:IMG 0934.JPG|RTENOTITLE]]<br />
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'''Miniprep of 3196sub_3194pcrclone'''<br />
&nbsp;3194 miniprep: Nhe1-KpnI digestion <br />
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[[File:IMG 0951.JPG|RTENOTITLE]] <br />
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3194 #6 could be good<br />
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[[Category:Cloning]]</div>
Paul.xu