https://eln.excellgen.com/index.php?action=history&feed=atom&title=3197_pT7A3-GST-BCMA 2026-10-18T19:24:23Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3197_pT7A3-GST-BCMA&diff=18688&oldid=prev 2018-07-13T18:04:44Z

<p></p> <p><b>New page</b></p><div>&#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 0858.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;PCR of 3197&#039;&#039;&#039;<br /> <br /> [[File:IMG 0849.JPG|RTENOTITLE]]<br /> <br /> <br /> <br /> &#039;&#039;&#039;Miniprep digestion of 2503_3122_3197_3191&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 2503<br /> | 1-4<br /> | Nde1+Xho1<br /> | 951bp<br /> | #1,2 seems good<br /> |-<br /> | 3122<br /> | 5-8<br /> | Nde1+Xho1<br /> | 1743bp<br /> | #5,6,8 seems good<br /> |-<br /> | 3197<br /> | 9-12<br /> | BamH1+Nco1<br /> | 1 band<br /> | may not good<br /> |-<br /> | 3191<br /> | 13-16<br /> | BamH1+Nco1<br /> | 722bp<br /> | #14,15,16 seems good<br /> |}<br /> <br /> [[File:IMG 0872.JPG|RTENOTITLE]]<br /> <br /> not good<br /> <br /> == &#039;&#039;&#039;Cloning of 3197 pT7A3-GST-BCMA&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3197 pT7A3-GST-BCMA<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;06, 22, 2018<br /> <br /> &#039;&#039;&#039;Notebook: 008840&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:008956&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3197&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> === Cloning ===<br /> <br /> &#039;&#039;&#039;Vector digestion_1000_3206_3197&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Vector is 1851&#039;&#039;&#039;<br /> <br /> [[File:IMG 0994.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;PCR screening of 3197&#039;&#039;&#039;<br /> <br /> [[File:IMG 1009.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 1002.JPG|RTENOTITLE]]<br /> <br /> sequencing result: all are empty vector<br /> <br /> <br /> <br /> = Redo =<br /> <br /> == PCR ==<br /> <br /> 6/27/2018<br /> <br /> [[File:3197 PCR.JPG|RTENOTITLE]]<br /> <br /> by yijia<br /> <br /> <br /> <br /> == Miniprep diegstion ==<br /> <br /> Enzymes:BamH1+BgIII<br /> <br /> Vector is 3225<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | Enzymes<br /> | Size<br /> | Note<br /> |-<br /> | 3197<br /> | 1-4<br /> | BamH1+BglII<br /> | 884<br /> | Maybe good. 3197-1--3197-4<br /> |}<br /> <br /> [[File:IMG 1049.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;redo 3197 PCR&#039;&#039;&#039;<br /> <br /> [[File:IMG 1112.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3197 miniprep digestion&#039;&#039;&#039;<br /> <br /> [[File:IMG 1120.JPG]]<br /> <br /> <br /> &#039;&#039;&#039;3197 pcr SCREENING&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | Lane<br /> | 72oC annealing<br /> | Note<br /> | &lt;br/&gt;<br /> |-<br /> | 3197<br /> | 1-8<br /> | 15s<br /> | #3,#4,#6,#7<br /> | &lt;br/&gt;<br /> |-<br /> | control<br /> | v1851;v3225;&amp;nbsp; &amp;nbsp;&#039;&#039;&#039;-----&amp;nbsp;&#039;&#039;&#039;no template<br /> | 15s<br /> | &lt;br/&gt;<br /> | &lt;br/&gt;<br /> |}<br /> <br /> [[File:IMG 1122.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div>

Crm