https://eln.excellgen.com/index.php?action=history&feed=atom&title=3200_pEPD 2026-10-18T15:58:14Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3200_pEPD&diff=18075&oldid=prev 2018-05-31T22:34:45Z

<p></p> <p><b>New page</b></p><div>5/26/2018<br /> <br /> 3200 miniprep digest with BmaH1+ NcoI + Xma1<br /> <br /> [[File:3200 digestion1.JPG|RTENOTITLE]]<br /> <br /> 5/27/2018<br /> <br /> digest with BamH1 + BglII or XmaI + NcoI<br /> <br /> [[File:3200 digestion2.JPG|RTENOTITLE]]<br /> <br /> [[File:3200 digestion3.JPG|RTENOTITLE]]<br /> <br /> Sample #2, 5, 6 are good by restriction analysis<br /> <br /> Send sample #6&amp;nbsp;to sequencing, good<br /> <br /> SfiI digestion, 40 min, FastDigest, Anza, CutSmart, 50 oC<br /> <br /> Looks like 40 min is not enough. CutSmart is the best<br /> <br /> [[File:3224 3200.JPG|RTENOTITLE]]<br /> <br /> Digest plasmid 300ng with SfiI o/n at 50 oC&amp;nbsp;<br /> <br /> [[File:3200 SfiI digestion.JPG]]</div>

Crm