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3206 pEF1-HTLV-LAMP-1 - Revision history
2026-10-18T08:18:20Z
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2018-08-09T21:00:52Z
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<p><b>New page</b></p><div>== '''Cloning of 3206 pEF1-HTLV-LAMP-1''' ==<br />
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'''Purpose:''' Cloning of 3206 pEF1-HTLV-LAMP-1<br />
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'''Date: '''06, 22, 2018<br />
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'''Notebook: 008840'''<br />
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'''Page:008956'''<br />
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'''Clone ID: 3206'''<br />
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'''Experimenters: '''yijia<br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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PCR from 987 pANT7_cGST-LAMP1<br />
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'''Vector digestion_1000_3206_3197'''<br />
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Vector is 3165<br />
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[[File:IMG 0994.JPG|RTENOTITLE]]<br />
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'''1st PCR of 3206'''<br />
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[[File:IMG 0985.JPG|RTENOTITLE]]<br />
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'''2nn PCR of 3206'''<br />
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[[File:IMG 0991.JPG|RTENOTITLE]]<br />
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=== &nbsp;Colony screen ===<br />
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'''Miniprep diegstion of 3206'''<br />
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[[File:IMG 1024.JPG|RTENOTITLE]]<br />
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need to do PCR screening<br />
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== Redo ==<br />
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'''3206 1st PCR'''<br />
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'''Template:987'''<br />
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Fragment 1: 3206-F1 + 988-R1<br/>Fragment 2: 988-F3 + 3206-R3<br />
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5X enhancer,homemade phusion, 5X thermo phusion HF buffer<br />
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[[File:IMG 1109.JPG|RTENOTITLE]]<br />
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'''3206 2nd PCR'''<br />
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'''Template:1st PCR above'''<br />
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Fragment 1: 3206-F1 + 3206-R4<br />
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Fragment 2: 3206-F2 + 3206-R3<br />
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5X enhancer,homemade phusion, 5X thermo phusion HF buffer<br />
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[[File:IMG 1115.JPG|RTENOTITLE]]<br />
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== '''Result intepretation/Summary''' ==<br />
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'''3206 PCR Screening'''<br />
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[[File:IMG 1284.JPG|RTENOTITLE]]<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]<br />
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[[Category:Cloning]]</div>
Crm