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3210 pT7A30-Sumo-Ov-ASP1-BCMA - Revision history
2026-10-18T08:19:37Z
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Crm at 18:47, 13 July 2018
2018-07-13T18:47:08Z
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<p><b>New page</b></p><div>'''Miniprep of 3122_3210'''<br />
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[[File:IMG 0902.JPG|RTENOTITLE]]<br />
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== Date:6/13/2018 ==<br />
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'''3210 subclone'''<br />
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[[File:IMG 0932.JPG|RTENOTITLE]]<br />
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'''Miniprep of 3210'''<br />
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[[File:IMG 0946.JPG|RTENOTITLE]]<br />
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Need to do verfication for clone #4<br/>Nde1-EcoR1: 1 kb<br/>BamH1+ Nde1: 363 bp<br/>BamH1 + Xho1; 613 bp<br />
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== Redo ==<br />
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'''Miniprep digestion_3196_3199_3210'''<br />
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'''Lane 16 is 3224pminiT2 as + control'''<br />
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[[File:IMG 1034.JPG|RTENOTITLE]]<br />
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== Date:7/6/2018 ==<br />
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redo<br />
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'''vector digestion test'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Enzymes<br />
| Result<br />
| Note<br />
|-<br />
| 1727<br />
| 1-3<br />
| Nde1+Xho1<br />
| transforma to make new miniprep<br />
| The vector from miniprep and GS maybe degrade, 3 is original plasmid, its a single band.<br />
|-<br />
| 1851<br />
| 4-5<br />
| Nde1+HindIII<br />
| need enough conc<br />
| 4 is 3191 from 1851 as vector<br />
|}<br />
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[[File:IMG 1089.JPG|RTENOTITLE]]<br />
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'''3210 subclone'''<br />
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[[File:IMG 1081.JPG|RTENOTITLE]]<br />
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'''Vector digestion of 3210'''<br />
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'''Vector is 2289 Nde1+EcoR1'''<br />
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[[File:IMG 1096.JPG|RTENOTITLE]]<br />
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'''miniprep digestion 3210'''<br />
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[[File:IMG 1119.JPG|RTENOTITLE]]<br />
[[Category:Cloning]]</div>
Crm