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3215 pCMV5-MICA-a-Avi-6H-BirA - Revision history
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Crm at 18:59, 25 July 2018
2018-07-25T18:59:01Z
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<p><b>New page</b></p><div>== '''Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA''' ==<br />
<br />
'''Purpose:''' Cloning of 3215 pCMV5-MICA-a-Avi-6H-BirA<br />
<br />
'''Date: '''06, 20, 2018<br />
<br />
'''Notebook:&nbsp;'''<br />
<br />
'''Page:'''<br />
<br />
'''Clone ID: 3215'''<br />
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'''Experimenters: '''yijia<br />
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== '''Results''' ==<br />
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=== Cloning ===<br />
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PCR from 3213 pANT7_cGST-MICA<br />
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'''PCR of 3215'''<br />
<br />
[[File:IMG 0965.JPG|RTENOTITLE]]<br />
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'''Vector digestion'''<br />
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Vector is 3192<br />
<br />
[[File:IMG 0957.JPG|RTENOTITLE]]<br />
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'''3215subclone'''<br />
<br />
[[File:IMG 1018.JPG|RTENOTITLE]]<br />
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=== &nbsp;Colony screen ===<br />
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'''Miniprep of 3215_3204'''<br />
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{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Vector<br />
| Clone # Name<br />
| Method<br />
| Enzymes<br />
| Size<br />
|-<br />
| 3215<br />
| 1-5<br />
| 3192<br />
| 1-5<br />
| PCR<br />
| Nhe1+Kpn1<br />
| 285<br />
|-<br />
| 3204<br />
| 6-10<br />
| 1393<br />
| 1-5<br />
| PCR<br />
| Nhe1+Kpn1<br />
| 564<br />
|}<br />
<br />
[[File:IMG 0971.JPG|RTENOTITLE]]<br />
<br />
'''Miniprep digestion _3204_3215_941'''<br />
<br />
Vector is 3237<br />
<br />
[[File:IMG 1032.JPG|RTENOTITLE]]<br />
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== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Crm