https://eln.excellgen.com/index.php?action=history&feed=atom&title=3218_pT7A30-ULP1 3218 pT7A30-ULP1 - Revision history 2026-10-18T13:21:33Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3218_pT7A30-ULP1&diff=18827&oldid=prev Crm at 20:51, 25 July 2018 2018-07-25T20:51:09Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of 3218 pT7A30-ULP1&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039; Cloning of 3218 pT7A30-ULP1<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;06, 22, 2018<br /> <br /> &#039;&#039;&#039;Notebook: 008840&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:008956&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID: 3218&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters: &#039;&#039;&#039;yijia<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === Cloning ===<br /> <br /> PCR from 2209 ZM552-ULP1<br /> <br /> &#039;&#039;&#039;1st PCR fo 3218&#039;&#039;&#039;<br /> <br /> [[File:IMG 0989.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;2nd PCR of 3218&#039;&#039;&#039;<br /> <br /> [[File:IMG 0999.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Vector digestion&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Vector digestion&amp;3225 subclone&#039;&#039;&#039;<br /> <br /> [[File:IMG 1001.JPG|RTENOTITLE]]<br /> <br /> === &amp;nbsp;Colony screen ===<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 3218&#039;&#039;&#039;<br /> <br /> [[File:IMG 1024.JPG|RTENOTITLE]]<br /> <br /> == redo ==<br /> <br /> &#039;&#039;&#039;2nd PCR 3218&#039;&#039;&#039;<br /> <br /> [[File:IMG 1087.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 3218&#039;&#039;&#039;<br /> <br /> [[File:IMG 1073.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;Miniprep digestion of 3218_998&#039;&#039;&#039;<br /> <br /> {| border=&quot;1&quot; cellspacing=&quot;1&quot; cellpadding=&quot;1&quot; style=&quot;width:500px;&quot;<br /> |-<br /> | #<br /> | lane<br /> | Enzymes<br /> | Size<br /> | Result<br /> |-<br /> | 3218<br /> | 1-8<br /> | HindIII<br /> | 617bp<br /> | no good<br /> |}<br /> <br /> [[File:IMG 1101.JPG|RTENOTITLE]]<br /> <br /> = 2nd Redo =<br /> <br /> &#039;&#039;&#039;1st PCR of 3218&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Template:2209&#039;&#039;&#039;<br /> <br /> F1:3218-F3+3218-R4<br /> <br /> F2:3218-F4+3218-R1<br /> <br /> 5X enhancer, homemade phusion, 5x thermo phusion HF buffer<br /> <br /> [[File:IMG 1109.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;2nd PCR of 3218&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Template:1st PCR above&#039;&#039;&#039;<br /> <br /> F1:3218-F1+3218-R1<br /> <br /> F2:3218-F2+3218-R2<br /> <br /> 5X enhancer, homemade phusion, 5x thermo phusion HF buffer<br /> <br /> [[File:IMG 1116.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;3218 miniprep digestion&#039;&#039;&#039;<br /> <br /> 3218--#9 need to confirm<br /> <br /> [[File:IMG 1155.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3218-#8,,same thing as #9 above,PCR screening&#039;&#039;&#039;<br /> <br /> [[File:IMG 1206.JPG|RTENOTITLE]]<br /> <br /> &#039;&#039;&#039;3218-8 digetsion&#039;&#039;&#039;<br /> <br /> [[File:IMG 1207.JPG|RTENOTITLE]]<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Crm