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3225 pT7A3-GST-MICA - Revision history
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2018-07-27T20:27:22Z
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<p><b>New page</b></p><div>== '''Cloning of 3225 pT7A3-GST-MICA''' ==<br />
<br />
'''Purpose: Cloning of 3225 pT7A3-GST-MICA'''<br />
<br />
'''Date: '''06, 22, 2018<br />
<br />
'''Notebook: 008840'''<br />
<br />
'''Page:008956'''<br />
<br />
'''Clone ID: 3225'''<br />
<br />
'''Experimenters: '''yijia<br />
<br />
== '''Results''' ==<br />
<br />
=== Cloning ===<br />
<br />
'''Vector digestion&3225 subclone'''<br />
<br />
'''vector is 1851;Nhe1+Xho1'''<br />
<br />
[[File:IMG 1001.JPG|RTENOTITLE]]<br />
<br />
'''Miniprep of 3225'''<br />
<br />
'''Enzymes:Nhe1+Xho1'''<br />
<br />
'''Size:383bp'''<br />
<br />
[[File:IMG 1025.JPG|RTENOTITLE]]<br />
<br />
actually is junk&nbsp;<br />
<br />
== '''redo''' ==<br />
<br />
'''miniprep digestion 3225_999_931'''<br />
<br />
{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"<br />
|-<br />
| #<br />
| Lane<br />
| Vector<br />
| Enyzmes<br />
| Size<br />
| note<br />
|-<br />
| 3225<br />
| 1-4; 5 is + control<br />
| 1851 pT7A3-GST<br />
| Nhe1+Xho1<br />
| 383bp<br />
| <br/><br />
|-<br />
| 999<br />
| 6-9;10 is +control<br />
| 1873<br />
| Nde1+Xho1<br />
| 1182bp<br />
| <br/><br />
|-<br />
| 931<br />
| 11-14;15 is + control<br />
| 931-1<br />
| HindIII+Xho1<br />
| <br/><br />
| failed<br />
|}<br />
<br />
[[File:IMG 1211.JPG|RTENOTITLE]]<br />
<br />
=== &nbsp;Colony screen ===<br />
<br />
== '''Result intepretation/Summary''' ==<br />
<br />
== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Crm