https://eln.excellgen.com/index.php?action=history&feed=atom&title=3231_pEPD-DP47-DPK22 3231 pEPD-DP47-DPK22 - Revision history 2026-10-18T07:50:16Z Revision history for this page on the wiki MediaWiki 1.44.0 https://eln.excellgen.com/index.php?title=3231_pEPD-DP47-DPK22&diff=18576&oldid=prev Paul.xu at 13:28, 3 July 2018 2018-07-03T13:28:52Z <p></p> <p><b>New page</b></p><div>== &#039;&#039;&#039;Cloning of&#039;&#039;&#039; ==<br /> <br /> &#039;&#039;&#039;Purpose:&#039;&#039;&#039;&amp;nbsp;&amp;nbsp;<br /> <br /> &#039;&#039;&#039;Date: &#039;&#039;&#039;2018-6-22<br /> <br /> &#039;&#039;&#039;Notebook:&amp;nbsp;&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Page:&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Clone ID:3237&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;Experimenters:&#039;&#039;&#039;&amp;nbsp;Paul&amp;nbsp;<br /> <br /> == &#039;&#039;&#039;Results&#039;&#039;&#039; ==<br /> <br /> === &#039;&#039;&#039;PCR&#039;&#039;&#039; ===<br /> <br /> Date:&amp;nbsp;Date: 6/21/2018<br /> <br /> By: Paul<br /> <br /> template:<br /> <br /> primers:<br /> <br /> Gel:<br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Cloning&#039;&#039;&#039;<br /> <br /> By: Paul<br /> <br /> Vector:&amp;nbsp;&amp;nbsp;<br /> <br /> Ligation: 30 min with T4 DNA ligase<br /> <br /> Competent cells: NEBTurbo<br /> <br /> <br /> <br /> &#039;&#039;&#039;Colony PCR Screening&amp;nbsp;&#039;&#039;&#039;<br /> <br /> <br /> <br /> ~&amp;nbsp;<br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Restriction Digestion&#039;&#039;&#039;<br /> <br /> Date: 6/~/2018<br /> <br /> By: Paul<br /> <br /> http://www.excellgen.net/eln/images/8/8c/3208_3231_3230_digestion.JPG<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> &#039;&#039;&#039;Sequencing&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:3230 fix restriction digestion.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:3230 3231 digestion.JPG|RTENOTITLE]]&#039;&#039;&#039; new 3231, digest with BamH1 -Xho1<br /> <br /> 7/2/18<br /> <br /> &#039;&#039;&#039;[[File:3231 BamH1 Xho1.JPG|RTENOTITLE]]&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;[[File:3231 Pst1 Xho1.JPG]]&#039;&#039;&#039;<br /> <br /> &#039;&#039;&#039;clone #2: sequencing is good&#039;&#039;&#039;<br /> <br /> == &#039;&#039;&#039;Result intepretation/Summary&#039;&#039;&#039; ==<br /> <br /> == &#039;&#039;&#039;Conclusion&#039;&#039;&#039; ==<br /> [[Category:Cloning]]</div> Paul.xu