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3232 pCMV5-Avi-6H-BirA - Revision history
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Crm at 17:57, 27 June 2018
2018-06-27T17:57:50Z
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<p><b>New page</b></p><div>== Dat3:6/8/2018 ==<br />
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'''PCR of 3232'''<br />
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[[File:IMG 0920.JPG|RTENOTITLE]]<br />
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'''Vector digestion of 3232'''<br />
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[[File:IMG 0924.JPG|RTENOTITLE]]<br />
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====== ========= ======<br />
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== '''Cloning of''' ==<br />
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'''Purpose:'''&nbsp;&nbsp;<br />
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'''Date: '''2018-6-22<br />
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'''Notebook:&nbsp;'''<br />
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'''Page:'''<br />
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'''Clone ID:3237'''<br />
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'''Experimenters:'''&nbsp;Paul&nbsp;<br />
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== '''Results''' ==<br />
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=== '''PCR''' ===<br />
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Date:&nbsp;Date: 6/21/2018<br />
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By: Paul<br />
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template:<br />
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primers:<br />
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Gel:<br />
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http://www.excellgen.net/eln/images/5/52/3237_3232_3234_PCR.JPG<br />
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'''Cloning'''<br />
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By: Paul<br />
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Vector:&nbsp;&nbsp;<br />
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Ligation: 30 min with T4 DNA ligase<br />
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Competent cells: NEBTurbo<br />
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'''Colony PCR Screening&nbsp;'''<br />
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'''Restriction Digestion'''<br />
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Date:&nbsp; 6/23/19, by Paul<br />
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http://www.excellgen.net/eln/images/2/2a/3237_3232_3234_minipre_digestion.JPG<br />
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'''Sequencing, #1 is good'''<br />
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== '''Result intepretation/Summary''' ==<br />
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== '''Conclusion''' ==<br />
[[Category:Cloning]]</div>
Crm